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Determinado por la plantilla, tasa variable de alargamiento de la cadena de ARN

D R Mills, C Dobkin, F R Kramer

|October 1, 1978

Resumen

La RNA polimerasa Q beta replicasa muestra tasas de alargamiento variables debido a sitios específicos de pausa. Estas pausas, probablemente causadas por estructuras de horquilla de ARN, pueden regular la síntesis de ácido nucleico.

Área de la Ciencia:

Biología Molecular Biología Molecular
La bioquímica es la bioquímica.

Sus antecedentes:

La enzima Q beta replicasa sintetiza el ARN MDV-1.
Se sabe que la tasa de polimerización del ARN varía.

Objetivo del estudio:

Investigar las causas de las tasas variables de polimerización del ARN.
Determinar el papel de las secuencias y estructuras específicas de ARN en la regulación de la actividad de la polimerasa.

Principales métodos:

Análisis electroforético de hebras de ARN parcialmente sintetizadas.
Análisis de la secuencia de nucleótidos de los intermediarios de alargamiento.

Principales resultados:

Se observaron tasas variables de alargamiento de la cadena de ARN.
Se identificaron sitios específicos de pausa, lo que lleva a la acumulación de ciertos productos intermedios.
Los productos intermedios prominentes mostraron potencial para la formación de horquilla.
Las estructuras de alfileres en las cadenas de plantillas o productos están implicadas en las pausas.

Conclusiones:

La formación de horquilla de ARN es una causa probable de pausas inducidas por la estructura durante la síntesis de ARN.
Estas pausas pueden ser un mecanismo general para regular la síntesis de ácido nucleico a través de varias polimerasas.

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