Video Experimental Relacionado
Updated: Aug 18, 2026
11:31
Generation of Recombinant Influenza Virus from Plasmid DNA
Published on: August 3, 2010
Recombinación de los virus de la gripe humana A en la naturaleza
Nature
|April 17, 1980
Resumen
Los virus de la influenza A (H1N1) y A (H2N2) circularon juntos en 1977, lo que generó preocupaciones sobre la recombinación genética. Este estudio identificó recombinancias genéticas entre los serotipos de influenza A (H1N1) y A (H3N1).
Área de la Ciencia:
- Virología Virología.
- Epidemiología La epidemiología.
- Genética La genética.
Sus antecedentes:
- En 1977, la gripe A (H1N1) reapareció, co-circulando con la gripe A (H2N2) en todo el mundo.
- La co-circulación y las infecciones mixtas sugirieron potencial para la recombinación viral.
- Análisis serológicos anteriores no pudieron detectar el reajuste antigénico (H2N1 o H1N2).
Objetivo del estudio:
- Para investigar la caracterización genética de los recombinantes del virus de la gripe.
- Para detectar recombinancias potenciales entre los serotipos H1N1 y H3N1, centrándose en los genes internos.
Principales métodos:
- Utilizó técnicas de hibridación ARN-ARN.
- Se analizaron aislamientos recientes de los serotipos influenzales A (H1N1) y A (H3N1).
Principales resultados:
- Se han detectado con éxito y caracterizado genéticamente recombinantes del virus de la influenza.
- Recombinantes identificados que involucran genes que no sean proteínas superficiales para ambos tipos antigénicos.
Conclusiones:
- Se produjo una recombinación genética entre cepas de virus de la influenza A en cocirculación.
- Este reordenamiento genético puede involucrar genes virales internos, no solo proteínas superficiales.
Videos de Conceptos Relacionados
00:57Viral Recombination
Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
00:36Viral Mutations
A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material for adaptive...
02:53Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
02:28Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
02:32Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
01:27Influenza
Influenza is an acute, highly communicable viral disease that affects the respiratory tract and is responsible for seasonal epidemics worldwide. Influenza A is the most prevalent type associated with widespread outbreaks and is subtyped based on two surface glycoproteins: hemagglutinin (H) and neuraminidase (N), as in H1N1. These glycoproteins are essential for viral infectivity, transmission, and immune recognition. Transmission occurs primarily through respiratory droplets and contaminated...