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Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
Published on: June 3, 2012
Secuencia de ARNm interrumpidos e ininterrumpidos y ADN clonado que codifica las dos proteínas no estructurales
Cell
|September 1, 1980
Resumen
El gen 8 del virus de la gripe produce dos proteínas, NS1 y NS2, a partir de distintos ARN mensajeros (ARNm). El ARNm NS2 contiene una secuencia interrumpida, lo que indica un evento de empalme similar a la expresión génica eucariota.
Área de la Ciencia:
- Virología Virología.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- El gen 8 del virus de la gripe codifica dos proteínas no estructurales, NS1 y NS2.
- Estudios anteriores indicaron que los ARNm NS1 y NS2 son co-terminales 3' y comparten un sitio poli (A).
Objetivo del estudio:
- Para determinar la secuencia completa de ADN del gen 8 del virus de la influenza (ADN NS).
- Para analizar la secuencia del ARNm NS2 y su relación con el ARNm NS1.
Principales métodos:
- Clonado y secuenciación de ADN NS de longitud completa del virus de la influenza.
- Secuenciación del ARN del ARNm NS2.
- Análisis de secuencias para identificar regiones de codificación, sitios de empalme y terminaciones de ARNm.
Principales resultados:
- El ARNm NS2 contiene una secuencia interrumpida de 473 nucleótidos, lo que sugiere un empalme.
- Se identificaron secuencias de nucleótidos conservados en las uniones de empalme, similares a los sitios de empalme de ARNm eucariotas.
- Tanto los ARNm NS1 como los NS2 comparten una secuencia 5' líder común y un codón de iniciación.
- Las proteínas NS1 y NS2 se superponen por 70 aminoácidos, traducidos desde diferentes marcos de lectura.
Conclusiones:
- El gen 8 del virus de la gripe utiliza un procesamiento alternativo de ARNm, incluido el empalme, para la expresión de proteínas.
- La secuencia de codificación superpuesta sugiere un mecanismo para generar diversidad de proteínas a partir de un solo segmento genético.
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