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Identificación del gen y el ARNm para el precursor de la proteína terminal del adenovirus

B W Stillman, J B Lewis, L T Chow

|February 1, 1981

Resumen

La replicación del ADN del adenovirus implica un precursor de proteína terminal de 87K que inicia la síntesis del ADN. Este precursor es procesado en la proteína terminal 55K durante la maduración del virus, posiblemente a través de un intermediario 62K, por la proteasa Ad2ts1.

Área de la Ciencia:

Biología Molecular Biología Molecular
Virología Virología.
La bioquímica es la bioquímica.

Sus antecedentes:

La replicación del ADN del adenovirus es iniciada por una proteína vinculada covalentemente al ADN.
El precursor de la proteína terminal del adenovirus 55K es una proteína 87K.
El ARNm para el precursor 87K se expresa temprano y tarde durante la infección.

Objetivo del estudio:

Para aclarar el papel de la proteína 87K en la replicación del ADN del adenovirus.
Para entender el procesamiento del precursor 87K a la proteína terminal madura 55K.
Para identificar la proteasa viral responsable del procesamiento.

Principales métodos:

Análisis de hibridación de ARN virales.
Análisis heterodúplex microscópico de electrones.
Análisis de péptidos de las proteínas virales.
Ensayos de replicación del ADN in vitro. ensayos de replicación del ADN in vitro.
Análisis de los viriones TS1 mutantes del adenovirus.

Principales resultados:

La proteína 87K es el producto de traducción primario y es primaria en la síntesis de ADN.
Una nueva región temprana del adenovirus está definida por ARN que codifican el precursor 87K y las proteínas relacionadas.
El precursor 87K es procesado a la proteína terminal 55K, potencialmente a través de un intermediario 62K, por la proteasa Ad2ts1.
Los viriones de tipo salvaje contienen la forma 55K, mientras que los viriones mutantes ts1 contienen la forma 87K a temperaturas restrictivas.

Conclusiones:

La proteína 87K sirve como precursora de la proteína terminal del adenovirus y es esencial para iniciar la replicación del ADN.
La maduración del virus implica el procesamiento proteolítico del precursor 87K a la proteína terminal 55K.
La proteasa Ad2ts1 juega un papel en el procesamiento de la proteína terminal durante la maduración del adenovirus.

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