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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
Published on: September 20, 2018
Represor de la transcripción eucariota con actividad de carboxipeptidasa
1Department of Biochemistry, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada.
Nature
|November 2, 1995
Resumen
Los investigadores identificaron AEBP1, un nuevo factor de transcripción crucial para la diferenciación de los adipocitos. AEBP1 reprime la expresión génica adiposa y puede regular la transcripción a través de la escisión enzimática.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La diferenciación de los adipocitos implica la regulación de los genes del metabolismo de los triglicéridos, como el gen adiposo P2 (aP2) que codifica ALBP.
- La secuencia AE-1 del promotor aP2 es un elemento regulador unido por factores que influyen en la expresión génica aP2.
Objetivo del estudio:
- Clonar y caracterizar el factor de preadipocitos 3T3 (AEBP1) implicado en la represión del gen aP2.
- Investigar el papel de AEBP1 y su actividad asociada a la carboxipeptidasa en la diferenciación de adipocitos.
Principales métodos:
- La clonación del ADN complementario (ADNc) para identificar el AEBP1.1.
- Análisis de la expresión de AEBP1 durante la diferenciación de los adipocitos.
- Evaluación de la actividad carboxipeptidasa de AEBP1 en la represión transcripcional.
Principales resultados:
- ADNc clonados que codifican el factor de preadipocitos 3T3 AEBP1.1.
- La expresión de AEBP1 se regula a la baja durante la diferenciación de los adipocitos.
- La actividad de la carboxipeptidasa asociada con AEBP1 es esencial para su función de represión transcripcional.
Conclusiones:
- AEBP1 actúa como un represor de la expresión génica aP2.
- AEBP1 puede representar una nueva clase de factores de transcripción que regulan la transcripción a través de la escisión enzimática.
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