Video Experimental Relacionado
Updated: Aug 9, 2026
07:55
Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment
Published on: September 25, 2017
La señalización polipeptídica al núcleo a través de la fosforilación de tirosina de las proteínas Jak y Stat
K Shuai1, A Ziemiecki, A F Wilks
1Laboratory of Molecular Cell Biology, Rockefeller University, New York 10021-6399.
Nature
|December 9, 1993
Resumen
Los interferones (IFN) desencadenan el transductor de señales y el activador de la activación de la proteína de transcripción (Stat) a través de la fosforilación de la tirosina. Este estudio sugiere que la Janus quinasa 1 (Jak1) puede ser la enzima clave que fosforila a Stat91 en respuesta a las IFN y al FEAG.
Área de la Ciencia:
- Las vías de señalización celular.
- Biología molecular La biología molecular.
- Inmunología Inmunología.
Sus antecedentes:
- Los interferones (IFN) se unen a los receptores de la superficie celular, iniciando la transducción de la señal.
- La fosforilación de tirosina de los transductores de señales y los activadores de las proteínas de transcripción (Stat) es un evento temprano clave.
- Las tirosinacinasas específicas, la tirosinacinasa-2 (Tyk2) y la Janus kinasa 2 (Jak2), están implicadas en la señalización IFN-alfa y IFN-gamma, respectivamente.
Objetivo del estudio:
- Para investigar los eventos de fosforilación de la tirosina en las proteínas Stat y Jak.
- Determinar el papel de las quinasas específicas en las vías de señalización del interferón y del factor de crecimiento epidérmico (EGF).
Principales métodos:
- Tratamiento celular con IFN-alfa, IFN-gamma y EGF.
- Análisis de la fosforilación de la tirosina en las proteínas Stat y Jak.
- Comparación de la activación de la quinasa por diferentes ligandos.
Principales resultados:
- Stat91 es fosforilado en Tyr701 por IFN-alfa, IFN-gamma y EGF.
- La Janus quinasa 1 (Jak1) es una tirosina fosforilada por los tres ligandos.
- Cada ligando activa al menos una cinasa distinta.
Conclusiones:
- Jak1 es una enzima candidata potencial para la fosforilación de Tyr701 en Stat91.
- Jak1 juega un papel en las vías de señalización activadas por las IFN y el FEAG.
- Las vías de señalización del interferón y el factor de crecimiento comparten componentes y mecanismos comunes.
Más Videos Relacionados
Videos de Conceptos Relacionados
01:19Interactions Between Signaling Pathways
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
01:15Amplifying Signals via Second Messengers
Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
01:22Amplifying Signals via Enzymatic Cascade
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
01:22PI3K/mTOR/AKT Signaling Pathway
The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...