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Epítopos exónicos de la variante CD44 en el cáncer de mama primario y duración de la supervivencia
M Kaufmann1, K H Heider, H P Sinn
1Department of Obstetrics and Gynaecology, University of Heidelberg, Germany.
Lancet (London, England)
|March 11, 1995
Resumen
La expresión del exón v6 de la variante CD44 en el cáncer de mama indica un mal pronóstico. Este epítopo CD44 es un predictor independiente significativo de la supervivencia en pacientes con tumores mamarios invasivos.
Área de la Ciencia:
- Oncología Oncología.
- Biología Molecular Biología Molecular
- Investigación de Investigación del Cáncer.
Sus antecedentes:
- CD44 es una familia de proteínas de la superficie celular producida por empalme alternativo.
- Las variantes de empalme que contienen secuencias de exones v6 están vinculadas a la metástasis en modelos de cáncer animal.
Objetivo del estudio:
- Para evaluar la expresión del epitopo del exón de la variante CD44 en el cáncer de mama humano.
- Para determinar el potencial pronóstico de estos epítopos en pacientes con cáncer de mama.
Principales métodos:
- Se utilizaron sueros policlonales purificados por afinidad y anticuerpos monoclonales contra las secuencias de exones de la variante humana CD44.
- Investigó la expresión de CD44 en 100 tumores mamarios invasivos primarios, recurrencias, metástasis y tejidos normales.
Principales resultados:
- Los exones de la variante CD44 (v3, v5, v6) se expresaron en la mayoría de los tumores, pero no en las células epiteliales normales del ducto mamario ni en las lesiones hiperplásticas.
- El epitopo DIII (exón v6) se encontró en el 84% de los tumores primarios y en el 100% de las metástasis.
- Los tumores exon v6-positivos se correlacionaron con una supervivencia general significativamente más baja (p=0,005).
Conclusiones:
- La expresión del exón de la variante CD44, particularmente el epítopo del exón v6, es un marcador pronóstico valioso en el cáncer de mama.
- El epítopo del exón v6 del CD44 es un predictor independiente del pronóstico, que no se ve afectado por el tamaño del tumor, el grado, el estado de los ganglios linfáticos o los niveles de receptores de progesterona.
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