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Antibody Transfection into Neurons as a Tool to Study Disease Pathogenesis
Published on: September 26, 2012
Base molecular de la imitación del antígeno por un anti-idiotopo
B A Fields1, F A Goldbaum, X Ysern
1Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville 20850, USA.
Nature
|April 20, 1995
Resumen
Los anticuerpos pueden imitar los antígenos a nivel molecular. El anticuerpo E5.2 contra la lisozima imita estructuralmente la lisozima.
Área de la Ciencia:
- Inmunología Inmunología.
- Biología Estructural Biología estructural.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Los idiótopos son determinantes únicos en los anticuerpos, formando el idiotipo.
- Los anticuerpos antiidiotípicos pueden imitar los antígenos externos, llevando una "imagen interna".
- Estudios anteriores carecían de evidencia estructural definitiva para esta imitación.
Objetivo del estudio:
- Para investigar la imitación molecular de antígenos por anticuerpos anti-idiotípicos.
- Para determinar la base estructural de las interacciones entre idiotopo y antiidiotopo.
- Para validar la imitación funcional a través de la inmunogenicidad.
Principales métodos:
- Cristalografía de rayos X de un complejo idiotopo-antiidiotopo (Fv fragmentos de D1.3 y E5.2).
- Análisis de las interacciones de los residuos en el sitio de unión.
- Inmunización con anticuerpo antiidiotípico E5.2.2.
Principales resultados:
- El anticuerpo anti-lizozima D1.3 se une a la lisozima y al anticuerpo anti-D1.3 E5.2 utilizando residuos similares en el sitio de combinación.
- E5.2 interactúa con D1.3 a través de contactos que imitan los de la lisozima.
- E5.2 induce una respuesta inmune anti-lizozima cuando se usa como inmunógeno.
Conclusiones:
- El anticuerpo antiidiotípico E5.2 imita estructuralmente a la lisozima en sus interacciones de unión.
- Esta imitación estructural apoya el concepto de anticuerpos de "imagen interna".
- Los anticuerpos antiidiotípicos pueden provocar respuestas inmunes específicas contra el antígeno original.
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