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La unión al FGF y la activación del receptor del FGF por di- y trisacáridos derivados de heparán sintético
D M Ornitz1, A B Herr, M Nilsson
1Department of Molecular Biology and Pharmacology, Washington University Medical School, St. Louis, MO 63110, USA.
Science (New York, N.Y.)
|April 21, 1995
Resumen
Los factores de crecimiento de los fibroblastos (FGF) necesitan heparina o sulfato de heparan (HS) para activar la señalización. Se encontró que los oligosacáridos HS pequeños y sintéticos activan el FGF, revelando sitios de unión y mecanismos para modular la actividad del FGF.
Área de la Ciencia:
- La bioquímica es la bioquímica.
- Biología Molecular Biología Molecular
- Biología Estructural Biología estructural.
Sus antecedentes:
- Los factores de crecimiento de los fibroblastos (FGF) son proteínas de señalización cruciales.
- Los FGF requieren heparina o sulfato de heparan (HS) como cofactor para la unión al receptor y la activación de la señal.
- Comprender el mecanismo molecular de la interacción FGF-heparina/HS es clave para controlar la señalización de FGF.
Objetivo del estudio:
- Investigar el mecanismo molecular por el cual la heparina/HS activa el FGF.
- Identificar las características estructurales específicas de la heparina/HS implicadas en la activación del FGF.
- Establecer una base para el diseño de moléculas que modulen la actividad del FGF.
Principales métodos:
- Análisis de oligosacáridos no sulfatados pequeños, sintéticos e isoméricos puros derivados de la heparina / HS para la actividad de señalización de FGF.
- Determinación de las estructuras cristalinas de los complejos FGF con estos oligosacáridos.
Principales resultados:
- Se descubrió que los oligosacáridos heparina/HS sintéticos activan la vía de señalización del FGF.
- Las estructuras cristalinas revelaron múltiples sitios de unión para estos oligosacáridos en el FGF.
- Estos hallazgos proporcionan información sobre la base estructural de la activación del FGF por heparina / HS.
Conclusiones:
- Los oligosacáridos de heparina/HS pueden activar directamente la señalización del FGF.
- Los sitios de vinculación del FGF identificados y los datos estructurales ofrecen un marco para comprender la activación del FGF.
- Esta investigación permite el diseño racional de nuevos compuestos para modular la actividad del FGF con fines terapéuticos o de investigación.
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