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Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
Published on: July 29, 2010
Activación de un gen de histona regulado por el ciclo celular por el factor de transcripción oncogénico IRF-2
P S Vaughan1, F Aziz, A J van Wijnen
1Department of Cell Biology, University of Massachusetts Medical Center, Worcester 01655, USA.
Nature
|September 28, 1995
Resumen
El gen FO108 de la histona H4 está regulado por el factor nuclear de la histona M (HiNF-M), identificado como factor regulador del interferón 2 (IRF-2). Esta proteína vincula la progresión del ciclo celular con la replicación del ADN, lo que afecta el crecimiento celular.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Regulación del ciclo celular Reglamento del ciclo celular.
- Expresión génica de la expresión génica.
Sus antecedentes:
- El gen FO108 de la histona H4 humana exhibe una transcripción dependiente del ciclo celular, que alcanza su punto máximo en la fase S temprana.
- La activación de los genes de la histona H4 requiere un elemento del ciclo celular (CCE) que se une a un factor proteico específico, el factor nuclear de la histona M (HiNF-M).
Objetivo del estudio:
- Para purificar HiNF-M e identificar su identidad molecular.
- Para aclarar el papel de HiNF-M en la regulación de la transcripción del gen histona H4 y su conexión con la progresión del ciclo celular.
Principales métodos:
- Se utilizaron ensayos de desplazamiento de movilidad electroforética (EMSA) para estudiar las interacciones proteína-ADN.
- Se realizó la purificación de HiNF-M.
- El factor regulador de interferón recombinante 2 (IRF-2) y el IRF-1 se utilizaron para probar su unión a la CCE y su efecto en la transcripción.
Principales resultados:
- HiNF-M fue purificado e identificado como el factor regulador del interferón 2 (IRF-2), un conocido regulador negativo de la respuesta al interferón.
- El IRF-2 y el IRF-1 recombinantes se unen específicamente a la CCE.
- IRF-2 e IRF-1 activan la transcripción del gen de la histona H4.
Conclusiones:
- El factor regulador del interferón 2 (IRF-2) es el factor proteico que se une al elemento del ciclo celular (CCE) y activa la transcripción del gen histona H4.
- IRF-2 tiene un papel funcional en el acoplamiento de la replicación del ADN y la progresión del ciclo celular en la transición de fase G1/S.
- Los hallazgos vinculan IRF-2, conocido por su potencial oncogénico, a un gen crítico involucrado en la proliferación celular.
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