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Clonado y expresión de TAFII250 humano: un factor asociado a TBP implicado en la regulación del ciclo celular
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Nature
|March 11, 1993
Resumen
Los investigadores identificaron hTAFII250, una proteína coactivadora clave esencial para la regulación de la transcripción génica. Este factor asociado a la TBP (TAF) vincula el control de la transcripción con la progresión del ciclo celular.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Regulación genética Reglamento genético.
- Biología celular Biología celular.
Sus antecedentes:
- La transcripción basal por la ARN polimerasa II requiere factores auxiliares (TFIIA-J).
- Los coactivadores son cruciales para la regulación del activador transcripcional, pero no para la transcripción basal.
- Algunos coactivadores se asocian con la proteína de unión a TATA (TBP) para formar el complejo TFIID.
Objetivo del estudio:
- Definir la relación entre la TBP y los factores asociados a la TBP (TAF).
- Para clonar, expresar y caracterizar el primer TAF humano, el hTAFII250.0.
Principales métodos:
- Clonado y expresión de la recombinación hTAFII250.0.
- Ensayos de unión in vitro y a base de levadura para evaluar la interacción de TBP.
- Análisis de la formación del complejo TFIID.
Principales resultados:
- El gen para hTAFII250 es idéntico al CCG1, un gen involucrado en la progresión del ciclo celular.
- El recombinante hTAFII250 se une directamente al TBP.
- hTAFII250 participa en la formación del complejo TFIID.
Conclusiones:
- hTAFII250 es el primer TAF humano identificado.
- Este TAF puede desempeñar un papel central en el ensamblaje de TFIID al interactuar con TBP y otros TAF.
- hTAFII250 vincula el control de la transcripción con el ciclo celular.
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