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Aislamiento de nuevos ribozimas de un gran grupo de secuencias aleatorias [ver comentario]
1Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Resumen
Los científicos descubrieron nuevos ARN catalíticos (ribozimas) que se unen de manera eficiente a las moléculas de ARN. Estas ribozimas, desarrolladas in vitro, exhiben tasas de ligadura millones de veces más rápidas que las reacciones no catalizadas.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
- La catálisis del ARN.
Sus antecedentes:
- Aislamiento de nuevos ARN catalíticos (ribozimas) mediante la selección iterativa in vitro de secuencias aleatorias.
- Caracterización de los ribozimas que catalizan la ligadura de ARN a través de un ataque 3'-hidroxilo en un 5'-trifosfato, imitando la síntesis de ARN de la enzima proteica.
- La reacción no catalizada también forma un enlace 3',5'-fosfodiéster.
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