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El virus Vaccinia codifica un receptor de interferón de tipo I soluble de nueva estructura y amplia especificidad de
J A Symons1, A Alcamí, G L Smith
1Sir William Dunn School of Pathology, University of Oxford, England.
Cell
|May 19, 1995
Resumen
El virus Vaccinia (VV) produce un receptor de interferón soluble único (B18R) que se une a interferones de tipo I de múltiples especies, ayudando a la replicación viral. La eliminación de este gen atenúa el VV en ratones.
Área de la Ciencia:
- Virología Virología.
- Inmunología Inmunología.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El virus Vaccinia (VV) y otros ortopoxvirus codifican proteínas para contrarrestar las respuestas inmunes del huésped.
- Los interferones tipo I (IFN) son cruciales para la inmunidad antiviral.
- Los receptores de citoquinas solubles son expresados por algunos virus para interferir con la inmunidad del huésped.
Objetivo del estudio:
- Para caracterizar el receptor de interferón de tipo I soluble codificado por el gen VV B18R.
- Investigar las propiedades de unión y el significado evolutivo de la proteína VV B18R.
- Para evaluar el papel de la proteína B18R en la virulencia VV.
Principales métodos:
- Caracterización bioquímica de la proteína B18R.
- Ensayos vinculantes para determinar la afinidad y la especificidad de especie para los IFN de tipo I.
- Generación y prueba de un mutante de deleción VV B18R en un modelo intranasal murino.
Principales resultados:
- El gen VV B18R codifica una glicoproteína de 60-65 kDa, un receptor IFN de tipo I soluble.
- Este receptor exhibe una alta afinidad por el IFN-alfa humano y una amplia especificidad de especie.
- Un mutante VV que carece de B18R mostró una virulencia reducida en un modelo de infección intranasal murina.
Conclusiones:
- La proteína B18R es un nuevo receptor IFN soluble de tipo I con una amplia especificidad de especie, que potencialmente facilita la replicación de VV entre huéspedes.
- B18R representa un importante mecanismo de evasión inmune viral.
- La proteína B18R es esencial para la virulencia completa de VV en un modelo murino.
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