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Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
Published on: January 19, 2012
El quimioatractor axonal netrina-1 también es un quimiorepelente para los axones motores trocleares
S A Colamarino1, M Tessier-Lavigne
1Howard Hughes Medical Institute, Department of Anatomy, University of California, San Francisco 94143-0452, USA.
Cell
|May 19, 1995
Resumen
Netrin-1, una molécula guía, actúa como quimioatractor y quimio-repelente para el desarrollo de axones. Esta acción dual guía el crecimiento neuronal en el tubo neural de los vertebrados, similar a su homólogo de C. elegans UNC-6.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
Sus antecedentes:
- La orientación de los axones es crucial para la formación de circuitos neuronales.
- Las moléculas difusibles actúan como quimioatractores y quimio repelentes.
- Netrin-1 es un conocido quimioatractor expresado por las células de la placa del suelo.
Objetivo del estudio:
- Para investigar el papel de la netrina-1 en la guía de los axones motores trochleares.
- Para determinar si la netrina-1 exhibe propiedades de guía bifuncional.
Principales métodos:
- Análisis in vivo de la orientación de los axones en el tubo neural en desarrollo.
- Utilizando las células COS para secretar netrina-1 recombinante.
Principales resultados:
- Las células de la placa del suelo ejercen un efecto repulsivo de largo alcance sobre los axones motores trochleares dorsales en crecimiento.
- La netrina-1 secretada por las células COS imita este efecto repulsivo.
- Netrin-1 demuestra un doble papel, atrayendo a algunos axones mientras repele a otros.
Conclusiones:
- Netrin-1 es una señal de guía bifuncional en el sistema nervioso de los vertebrados.
- Esta bifuncionalidad se conserva en todas las especies, como se ve con UNC-6 en C. elegans.
- Netrin-1 juega un papel crítico en el establecimiento de conexiones neuronales precisas.
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