Video Experimental Relacionado
Updated: Aug 18, 2026
16:24
Analyzing and Building Nucleic Acid Structures with 3DNA
Published on: April 26, 2013
La estructura cristalina del factor de transcripción bZIP heterodimérico c-Fos-c-Jun unido al ADN
1Howard Hughes Medical Institute, Harvard University, Cambridge, Massachusetts 02138.
Nature
|January 19, 1995
Resumen
Los factores de transcripción Fos y Jun forman heterodímeros que se unen al ADN. La cristalografía de rayos X revela su estructura, mostrando cómo estos complejos proteicos interactúan con los elementos de reconocimiento de ADN.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología Estructural Biología estructural.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las proteínas Fos y Jun son factores de transcripción de los eucariotas.
- Forman heterodímeros para unirse a secuencias específicas de ADN (5'-TGAGTCA-3').
Objetivo del estudio:
- Para determinar la estructura cristalina de rayos X de un heterodímero c-Fos/c-Jun unido al ADN.
- Para dilucidar las bases estructurales de la formación de heterodímeros Fos-Jun y la unión al ADN.
Principales métodos:
- Cristalografía de rayos X con rayos X.
- Análisis del complejo proteína-ADN.
Principales resultados:
- El heterodímero c-Fos/c-Jun se compone de hélices alfa continuas.
- La estructura de la bobina enrollada está flexiblemente vinculada a las regiones básicas de unión al ADN.
- El heterodimero no une el elemento de ADN en una sola orientación fija.
- Las extensas interacciones electrostáticas estabilizan el heterodimero sobre los homodimeros.
Conclusiones:
- La estructura proporciona información sobre los mecanismos moleculares de la función del factor de transcripción Fos-Jun.
- La flexibilidad en el enlace de la bobina en espiral permite el reconocimiento de ADN adaptable.
- Las interacciones electrostáticas son cruciales para la formación de heterodímeros preferenciales.
Más Videos Relacionados
Videos de Conceptos Relacionados
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:13Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
02:42Eukaryotic Transcription Activators
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:13Cooperative Binding of Transcription Regulators
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...