Video Experimental Relacionado
Updated: Aug 8, 2026
10:10
Dissection and Immunostaining of Imaginal Discs from Drosophila melanogaster
Published on: September 20, 2014
La proteína quinasa dependiente de cAMP y el erizo actúan antagónicamente en la regulación de la transcripción
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200.
Cell
|February 24, 1995
Resumen
La proteína quinasa dependiente de cAMP (PKA) reprime la expresión decapentaplegia (dpp) en el desarrollo de Drosophila. La pérdida de la actividad de PKA conduce a la dpp ectópica, influyendo en el destino celular y la formación de patrones en los discos imaginales.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética molecular La genética molecular.
- La señalización celular de las células.
Sus antecedentes:
- La decapentaplegia (dpp) es crucial para el desarrollo del disco imaginal de Drosophila.
- La regulación precisa de la expresión de dpp es esencial para la formación de patrones.
Objetivo del estudio:
- Para investigar el papel de la proteína quinasa dependiente de cAMP (PKA) en la regulación de la expresión de dpp.
- Para comprender cómo el PKA influye en el destino celular y la formación de patrones en los discos imaginarios de Drosophila.
Principales métodos:
- Utilizó mosaicos genéticos en Drosophila.
- Las células analizadas carecen de actividad de PKA en el apéndice y los discos del ojo.
- Evaluó la expresión ectópica del dpp y sus efectos en las células vecinas.
Principales resultados:
- Las células que carecen de actividad de PKA expresan ectopicamente dpp en el apéndice anterior y los compartimentos del disco ocular.
- Las células con deficiencia de PKA influyen en el destino celular vecino, alterando los patrones anteriores e induciendo surcos morfogenéticos ectópicos.
- La actividad organizativa de las células mutantes de Pka es dependiente de dpp.
Conclusiones:
- La PKA es un componente de una vía de señalización que reprime la expresión de la dpp.
- La señalización de Hedgehog (hh) se opone a esta vía, manteniendo la expresión de dpp en los límites de los compartimentos y el surco morfogenético.
Videos de Conceptos Relacionados
02:54Canonical Wnt Signaling Pathway
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
02:34Inhibition of Cdk Activity
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
01:41Non-Canonical Wnt Signaling Pathways
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
01:19Interactions Between Signaling Pathways
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
01:20The JAK-STAT Signaling Pathway
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...