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Tractable Mammalian Cell Infections with Protozoan-primed Bacteria
Published on: April 2, 2013
La expresión ectópica del regulón flagellar altera el desarrollo de la interacción Bordetella-anfitrión
B J Akerley1, P A Cotter, J F Miller
1Department of Microbiology and Immunology, School of Medicine, University of California, Los Angeles 90024.
Cell
|February 24, 1995
Resumen
El control de la virulencia de Bordetella (bvgAS) activa y reprime los genes. La represión de los genes de motilidad es crucial para la colonización del huésped, ya que su expresión durante la infección dificulta la infección bacteriana.
Área de la Ciencia:
- Microbiología Microbiología.
- La patogénesis bacteriana es la patogénesis bacteriana.
- Regulación genética Reglamento genético.
Sus antecedentes:
- Los sistemas de dos componentes son vitales para la adaptación bacteriana y la patogénesis.
- El locus Bordetella bvgAS regula los factores de virulencia en respuesta a las señales ambientales.
- El papel de la represión mediada por BvgAS durante la infección sigue sin estar claro.
Objetivo del estudio:
- Para investigar la función de los genes reprimidos por BvgAS durante la infección por Bordetella.
- Para determinar si los genes reprimidos son necesarios para la colonización del huésped.
- Para aclarar el doble papel de BvgAS en la promoción de la virulencia.
Principales métodos:
- Manipulación genética de los genes reguladores de Bordetella.
- Análisis de patrones de expresión génica bajo diferentes condiciones de crecimiento.
- Evaluación de la colonización bacteriana en un modelo de huésped.
Principales resultados:
- Los genes de motilidad reprimidos por BvgAS no son necesarios para la colonización del huésped.
- La expresión de los genes de motilidad durante la fase Bvg+ perjudica la colonización traqueal.
- BvgAS controla la virulencia mediante la activación de los factores de colonización y la represión de los factores inhibidores.
Conclusiones:
- La represión mediada por BvgAS es esencial para una patogénesis efectiva de Bordetella.
- La represión de genes no esenciales o perjudiciales mejora la colonización bacteriana.
- El locus bvgAS orquesta una compleja red reguladora para una infección exitosa.
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