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Una familia de proteínas adaptadoras de transcripción dirigidas por la oncoproteína E1A
Nature
|March 2, 1995
Resumen
La oncoproteína E1A adenoviral se dirige a las proteínas p300 y CBP, que son proteínas adaptadoras de la transcripción. El E1A inhibe el p300 y el p300A.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- Virología Virología.
Sus antecedentes:
- La proteína p300 regula el ciclo celular, los potenciadores y la diferenciación.
- La proteína CBP coactiva el factor de transcripción CREB y los promotores inducidos por la AMP cíclica.
- p300 y CBP comparten secuencias muy similares.
Objetivo del estudio:
- Investigar la relación funcional entre p300, CBP y E1A. adenoviral.
- Para determinar si E1A inhibe la actividad transcripcional de p300 y CBP.
- Para explorar la interacción in vivo entre E1A y CBP.
Principales métodos:
- Evaluación de la estimulación transcripcional por p300.
- Prueba del efecto inhibidor de E1A en la actividad de p300.
- Investigando la asociación de CBP con los miembros de la familia CREB vinculados al ADN.
- Examinando la interacción de E1A y CBP in vivo.
Principales resultados:
- p300 estimula la transcripción, y esta actividad es específicamente inhibida por E1A.
- CBP forma complejos ligados al ADN con los miembros de la familia CREB.
- E1A y CBP interactúan in vivo.
- E1A reprime la transcripción dependiente de la cAMP, lo que indica una focalización funcional de la CBP.
Conclusiones:
- p300 y CBP constituyen una familia de proteínas adaptadoras de la transcripción.
- La oncoproteína E1A adenoviral se dirige específicamente a esta familia de proteínas p300 / CBP.
- La interacción de E1A con CBP y la inhibición de la actividad transcripcional son mecanismos clave de la oncogénesis viral.
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