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Analysis of Protein-protein Interactions and Co-localization Between Components of Gap, Tight, and Adherens Junctions in Murine Mammary Glands
Published on: May 30, 2017
Análisis funcional de las activinas durante el desarrollo de mamíferos
M M Matzuk1, T R Kumar, A Vassalli
1Department of Molecular and Human Genetics, Howard Hughes Medical Institute, Baylor College of Medicine, Houston, Texas 77030.
Nature
|March 23, 1995
Resumen
Activin-beta Una deficiencia en ratones causa defectos craneofaciales y muerte prematura, revelando su papel crucial en el desarrollo. No se encontró redundancia funcional entre la activina-beta A y la activina-beta B durante la embriogénesis.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- Las activinas, miembros diméricos de la superfamilia TGF-beta, se conservan en los vertebrados.
- Están implicados en la inducción del mesodermo y la neurulación en los vertebrados inferiores.
Objetivo del estudio:
- Para investigar la función in vivo de las activinas de los mamíferos.
- Para determinar el papel de la activina-beta A y la activina-beta B durante la embriogénesis del ratón.
Principales métodos:
- Generación de ratones con mutaciones en los genes de la activina-beta A y la activina-beta B.
- Análisis de defectos del desarrollo en ratones mutantes.
Principales resultados:
- Los ratones con deficiencia de activina-beta A mueren dentro de las 24 horas posteriores al nacimiento, exhibiendo defectos craneofaciales como paladar hendido, bigote ausente e incisivos inferiores.
- Los ratones que carecen de ambas subunidades de activina muestran los defectos combinados de los mutantes individuales, lo que indica que no hay redundancia funcional.
- La expresión de la activina cigótica no es esencial para la formación del mesodermo del ratón, a diferencia de los vertebrados inferiores.
Conclusiones:
- La activina-beta A es esencial para el desarrollo craneofacial de los mamíferos.
- No hay redundancia funcional entre la activina-beta A y la activina-beta B durante la embriogénesis del ratón.
- Los roles de las activinas en la formación del mesodermo difieren entre los ratones y los vertebrados inferiores.
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