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Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Published on: May 27, 2016
La proteína nuclear CBP es un coactivador para el factor de transcripción CREBEB
R P Kwok1, J R Lundblad, J C Chrivia
1Vollum Institute, Oregon Health Sciences University, Portland 97201.
Nature
|July 21, 1994
Resumen
El factor de transcripción CREB se une al ADN, y su activación por la proteína quinasa A (PKA) implica el coactivador CBP. La CBP se une al phosphoCREB y activa la transcripción de los genes sensibles a la cAMP.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Reglamento genético Reglamento genético.
- Factores de transcripción Factores de transcripción
Sus antecedentes:
- CREB (proteína de unión del elemento de respuesta CAMP) se une a las secuencias de ADN CRE.
- La activación de CREB por fosforilación de PKA no se entiende completamente.
- El reclutamiento de coactivadores es un mecanismo propuesto para la activación de CREB.
Objetivo del estudio:
- Investigar la interacción entre el CREB fosforilado (phosphoCREB) y el coactivador CBP.
- Para caracterizar los parámetros de unión del complejo fosfoCREB:CBP.
- Determinar el papel de la CBP en la activación transcripcional.
Principales métodos:
- Mediciones de anisotropía de fluorescencia para definir parámetros vinculantes.
- Análisis del dominio de activación carboxiterminal del CBP.
- Investigación de la interacción de CBP con el factor de transcripción basal TFIIB.
Principales resultados:
- CBP se une específicamente a la forma PKA-fosforilada de CREB.
- La CBP activa la transcripción a través de su terminal carboxínico.
- El dominio de activación de CBP interactúa con TFIIB, similar al coactivador de levadura ADA-1.
Conclusiones:
- La CBP funciona como un coactivador para el phosphoCREB.
- La CBP mejora la actividad transcripcional del phosphoCREB en los genes sensibles a la cAMP.
- La interacción entre el CBP y el TFIIB es crucial para la coactivación.
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