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FGF5 como regulador del ciclo de crecimiento del cabello: evidencia de mutaciones dirigidas y espontáneas
J M Hébert1, T Rosenquist, J Götz
1Department of Anatomy and Programs in Genetics and in Developmental Biology, School of Medicine, University of California, San Francisco 94143.
Cell
|September 23, 1994
Resumen
El factor de crecimiento de fibroblastos 5 (FGF5) inhibe el alargamiento del cabello. Los estudios genéticos en ratones revelan FGF5F5.
Área de la Ciencia:
- Genética La genética.
- Biología Molecular Biología Molecular
- Dermatología Dermatología dermatología.
Sus antecedentes:
- El factor de crecimiento de fibroblastos 5 (FGF5) es una proteína de señalización secretada que participa en varios procesos biológicos.
- La mutación espontánea angora (go) en ratones se caracteriza por el pelo anormalmente largo.
- La base genética y la función de FGF5 en la regulación del crecimiento del cabello no se comprendieron completamente.
Objetivo del estudio:
- Investigar el papel del factor de crecimiento de fibroblastos 5 (FGF5) en la regulación del crecimiento del cabello.
- Para identificar el gen responsable de la mutación de angora (go) en ratones.
- Para aclarar el mecanismo por el cual FGF5 influye en el ciclo del cabello.
Principales métodos:
- La orientación del gen en las células madre embrionarias para crear ratones mutantes fgf5neo.
- Análisis fenotípico de ratones homocigotos para las mutaciones fgf5neo y go.
- Pruebas de complementación entre las mutaciones fgf5neo y go.
- Análisis de ADN para identificar mutaciones en el gen Fgf5.
- Análisis de la expresión de Fgf5 en los folículos pilosos de ratón durante el ciclo de crecimiento del cabello.
Principales resultados:
- Los ratones con un alelo nulo predicho de Fgf5 (fgf5neo) exhibieron vello anormalmente largo.
- El fenotipo de los ratones fgf5neo era indistinguible de los ratones mutantes espontáneos de angora (go).
- Las mutaciones fgf5neo y go no se complementan, lo que indica que afectan al mismo gen.
- El exón 1 de Fgf5 fue eliminado en los homocigotos go, confirmando que go es un alelo mutante de Fgf5.5.
- La expresión de Fgf5 se detectó en los folículos pilosos de ratón de tipo salvaje, específicamente en la vaina externa de la raíz durante el anagén VI.
Conclusiones:
- FGF5 actúa como un inhibidor del alargamiento del cabello.
- La mutación de angora (go) es un alelo de pérdida de función del gen Fgf5.
- FGF5 juega un papel crucial en la regulación del ciclo de crecimiento del cabello mediante el control del alargamiento del cabello.
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