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Papel de la SAPK/ERK quinasa-1 en la vía activada por el estrés que regula el factor de transcripción c-Jun
I Sánchez1, R T Hughes, B J Mayer
1Diabetes Research Laboratory, Massachusetts General Hospital East, Charlestown.
Nature
|December 22, 1994
Resumen
Las proteínacinasas activadas por el estrés (SAPK) son cruciales para las respuestas al estrés celular. Este estudio identifica a la SAPK/ERK quinasa-1 (SEK1) como un nuevo activador aguas arriba, que define una vía de señalización clave desde el estrés hasta la activación génica.
Área de la Ciencia:
- La señalización celular de las células.
- Biología molecular La biología molecular.
- Las vías de respuesta al estrés.
Sus antecedentes:
- Las proteínacinasas activadas por el estrés (SAPK) son reguladores clave de las respuestas celulares a varios tipos de estrés.
- Las SAPKs son distintas de las proteínacinasas activadas por mitógenos (MAPKs), pero comparten cascadas de señalización relacionadas.
- La activación SAPK de c-Jun está implicada en la regulación de la transcripción génica.
Objetivo del estudio:
- Para identificar nuevas proteínas quinasas involucradas en la activación de SAPK.
- Para caracterizar el papel de una quinasa recién identificada, SAPK/ERK quinasa-1 (SEK1), en la señalización del estrés.
- Para dilucidar la cascada de señalización que vincula el estrés celular con el factor de transcripción c-Jun.
Principales métodos:
- Identificación y caracterización de una nueva proteína quinasa, SEK1.
- Ensayos in vitro e in vivo para evaluar la activación de las SAPKs por SEK1.
- Utilizando un mutante SEK1 inactivo para investigar su papel en el bloqueo de la activación de SAPK.
Principales resultados:
- SEK1 fue identificado como una nueva proteína quinasa estructuralmente relacionada con las MAP quinasa quinasas (MEKs).
- SEK1 demostró una activación potente de las SAPK tanto in vitro como in vivo.
- Un mutante SEK1 inactivo bloqueó efectivamente la activación de SAPK inducida por estímulos extracelulares sin afectar la vía MAPK.
Conclusiones:
- SEK1 es un importante activador aguas arriba de SAPKs, definiendo una vía de señalización crítica.
- Esta vía conecta las señales de estrés celular con la activación del factor de transcripción c-Jun.
- SEK1 proporciona un nuevo enlace molecular en la cascada de señalización inducida por el estrés.
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