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El factor de transcripción de la hélice alada HNF-3 beta es necesario para el desarrollo del notocordo en el embrión
D C Weinstein1, A Ruiz i Altaba, W S Chen
1Laboratory of Molecular Cell Biology, Rockefeller University, New York, New York 10021.
Cell
|August 26, 1994
Resumen
El factor nuclear hepatocitario 3 beta (HNF-3 beta) es crucial para el desarrollo temprano del ratón. Su ausencia impide la formación del nodo y el notocordo, lo que lleva a la letalidad embrionaria para el día 10-11.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
- Biología Molecular Biología Molecular
Sus antecedentes:
- El factor nuclear hepatocitario-3 beta (HNF-3 beta) es un factor de transcripción.
- Se expresa en células endodermales y de línea media embrionarias y adultas.
Objetivo del estudio:
- Para definir la función del HNF-3 beta en el desarrollo embrionario del ratón.
- Para investigar las consecuencias del desarrollo de la deficiencia de HNF-3 beta.
Principales métodos:
- Mutación dirigida del locus beta HNF-3 utilizando recombinación homóloga en células madre embrionarias.
- Análisis de embriones mutantes desde el día embrionario 6.5 al 10-11.
Principales resultados:
- Los ratones que carecen de HNF-3 beta muestran letalidad embrionaria entre los días 10 y 11.
- Los embriones mutantes no logran formar un nodo y un notocordio distintos.
- Se observaron defectos en la organización del tubo somático y del tubo neural, ausencia de placa de suelo y neuronas motoras, y fallo en la formación del tubo intestinal.
Conclusiones:
- HNF-3 beta juega un papel esencial en el desarrollo del mesodermo axial.
- El factor de transcripción es crítico para el establecimiento de estructuras embrionarias clave como el nodo y la notocorda.
- El HNF-3 beta es indispensable para la embriogénesis normal del ratón.
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