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Una proteína similar a la HMG que puede cambiar un activador transcripcional a un represor
N Lehming1, D Thanos, J M Brickman
1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Nature
|September 8, 1994
Resumen
La proteína DSP1 de Drosophila convierte los factores de transcripción Dorsal y NF-kappa B de activadores a represores. Este interruptor depende de un elemento regulador negativo, destacando las proteínas HMG.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología del desarrollo Biología del desarrollo.
- Genética La genética.
Sus antecedentes:
- La regulación transcripcional involucra proteínas que pueden activar o reprimir la expresión génica.
- La proteína Dorsal de Drosophila, un miembro de la familia Rel, activa la torsión y reprime el zen en los primeros embriones.
- El papel de las proteínas HMG en la modulación de la actividad transcripcional no se entiende completamente.
Objetivo del estudio:
- Identificar y caracterizar una proteína de Drosophila HMG1 involucrada en la regulación de la función dorsal.
- Investigar el mecanismo por el cual las proteínas HMG influyen en la activación y represión de genes.
- Para determinar si las proteínas HMG pueden cambiar los reguladores transcripcionales entre los modos de activación y represión.
Principales métodos:
- Identificación y clonación de una nueva proteína de Drosophila HMG1, DSP1.
- Análisis del efecto de DSP1 en la actividad de Dorsal y NF-kappa B en ensayos de expresión génica.
- Identificación y caracterización de un elemento regulador negativo (ERN) involucrado en la represión mediada por el DSP1.
Principales resultados:
- Se identificó una nueva proteína de Drosophila HMG1, DSP1.
- DSP1 convierte el activador transcripcional Dorsal en un represor.
- Esta represión depende de un elemento regulador negativo (ERN) ubicado cerca de los sitios de unión dorsal.
Conclusiones:
- DSP1 actúa como un interruptor, convirtiendo Dorsal y NF-kappa B de activadores de la transcripción a los represores.
- La interacción con un elemento regulador negativo (ERN) es crucial para este cambio.
- Las proteínas similares a las HMG, como DSP1 y HMG I(Y), juegan un papel crítico en la determinación del resultado transcripcional de las proteínas reguladoras.
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