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Operones en C. elegans: los precursores de ARNm policistrónico se procesan mediante trans-splicing de SL2 a las

J Spieth¹, G Brooke, S Kuersten

¹Department of Biology, Indiana University, Bloomington 47405.

|May 7, 1993

Resumen

Los operones de C. elegans utilizan el trans-splicing para la expresión génica aguas abajo. Los genes aguas abajo en los operones están empalmados a SL2, lo que indica que la cotranscripción regula el procesamiento del ARNm.

Área de la Ciencia:

Biología Molecular Biología Molecular
Genética La genética.
Biología del desarrollo Biología del desarrollo.

Sus antecedentes:

C. elegans expresión génica de la expresión génica.
El mecanismo de trans-espleaje.
La estructura del operón es la estructura del operón.

Objetivo del estudio:

Investigar el procesamiento genético aguas abajo en los operones de C. elegans.
Determinar el papel de la cotranscripción en el empalme del ARNm.
Caracteriza la regulación del trans-esplicado SL2.

Principales métodos:

clonación de ADNc clonación de ADNc
Secuenciación de ARN de secuenciación de ARN.
La manipulación genética (reubicación de genes)

Principales resultados:

Se han identificado genes upstream en los operones de C. elegans.
Se ha demostrado la cotranscripción de genes agrupados.
Los ARNm aguas abajo que se muestran se forman por escisión y trans-splicing.
Los ARN policistrónicos confirmados pueden ser procesados por trans-splicing.
Establecido trans-splicing SL2 como consecuencia de la ubicación del operón aguas abajo.

Conclusiones:

Los operones de C. elegans facilitan la cotranscripción y el trans-splicing.
La ubicación del gen aguas abajo dicta el empalme SL2.
El trans-splicing es un mecanismo regulador clave en la expresión génica de C. elegans.

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