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Identificación y secuencia de un factor accesorio requerido para la activación del receptor gamma de interferón
J Soh1, R J Donnelly, S Kotenko
1Department of Molecular Genetics and Microbiology, University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Piscataway 08854-5635.
Cell
|March 11, 1994
Resumen
El cromosoma 21 humano proporciona factores accesorios esenciales para la actividad del interferón gamma humano (Hu-IFN-gamma), incluida la protección antiviral. Estos factores, junto con el receptor en el cromosoma 6, son cruciales para una respuesta biológica completa a Hu-IFN-gamma.
Área de la Ciencia:
- Inmunología Inmunología.
- Genética La genética.
- Virología Virología.
Sus antecedentes:
- El interferón gamma humano (Hu-IFN-gamma) media respuestas inmunes críticas, incluida la inducción de antígenos HLA de clase I y la protección antiviral.
- La sensibilidad a Hu-IFN-gamma requiere información genética codificada en los cromosomas humanos 6 y 21.
Objetivo del estudio:
- Identificar y caracterizar los factores accesorios codificados por el cromosoma 21 humano que contribuyen a la actividad biológica Hu-IFN-gamma.
- Para aclarar los roles específicos de los factores codificados en el cromosoma 21 en la señalización del receptor Hu-IFN-gamma y la defensa antiviral.
Principales métodos:
- Utilizó sondas de clonación genómica para identificar clones de ADNc que expresan factores accesorios específicos de la especie.
- Evaluó la capacidad de los clones de ADNc identificados para reconstituir la inducción mediada por gamma Hu-IFN de los antígenos HLA de clase I.
- Se evaluó el impacto de estos clones de ADNc en la protección antiviral contra el virus de la encefalomiocarditis (EMCV).
Principales resultados:
- Clones de ADNc identificados que codifican un factor accesorio que puede sustituir al cromosoma 21 humano en la reconstitución de la inducción del antígeno HLA clase I.
- Se demostró que este factor por sí solo no confiere una protección antiviral completa contra el EMCV, lo que indica la necesidad de factores adicionales codificados en el cromosoma 21.
- Se ha confirmado que el cromosoma 21 humano codifica múltiples factores accesorios para distintas funciones Hu-IFN-gamma.
Conclusiones:
- El factor accesorio identificado es parte de una familia de factores que median diferentes acciones de Hu-IFN-gamma.
- El cromosoma 21 humano juega un papel complejo en la señalización Hu-IFN-gamma, codificando tanto los factores asociados al receptor como los factores esenciales para la actividad antiviral completa.
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