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Published on: June 2, 2016
La glicoproteína del virus de la estomatitis vesicular se clasifica y concentra durante la exportación desde el
W E Balch1, J M McCaffery, H Plutner
1Department of Cell Biology, Scripps Research Institute, La Jolla, California 92037.
Cell
|March 11, 1994
Resumen
Las proteínas recién sintetizadas se clasifican y se concentran en las vesículas durante la exportación del retículo endoplasmático (ER), desafiando el modelo de flujo masivo. Esta clasificación mediada por ER asegura una entrada eficiente de proteínas en la vía secretora.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología molecular La biología molecular.
- El tráfico de proteínas.
Sus antecedentes:
- Las proteínas se transportan desde el retículo endoplasmático (RE) al aparato de Golgi a través del transporte vesicular.
- El modelo prevaleciente sugiere que la clasificación de proteínas ocurre exclusivamente en la red trans-Golgi (TGN) y el transporte de ER a Golgi se basa en el flujo a granel.
Objetivo del estudio:
- Investigar el mecanismo de clasificación y concentración de proteínas durante la exportación de ER.
- Para determinar si la clasificación de proteínas se produce antes del aparato de Golgi.
Principales métodos:
- Se empleó microscopía inmunoelectrónica cuantitativa para rastrear el movimiento y la concentración de proteínas virales.
- La glicoproteína del virus de la estomatitis vesicular (VSV-G) se utilizó como proteína modelo para estudiar la dinámica de exportación de ER.
Principales resultados:
- Se observó que el VSV-G se clasificaba de las proteínas residentes de la ER durante el brote de las vesículas de la ER.
- Se detectó una concentración de 5 a 10 veces mayor de VSV-G en vesículas de 40-80 nm durante la salida de ER.
- Esta concentración en los portadores vesiculares pre-Golgi representa la etapa de concentración primaria para el transporte VSV-G.
Conclusiones:
- La exportación de ER no está mediada únicamente por el flujo a granel, lo que indica un proceso selectivo.
- El ER clasifica y concentra activamente las proteínas, asegurando una entrada eficiente en la vía secretora.
- Este estudio revela un nivel inesperado de control de ER sobre la exportación de proteínas.
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