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Una mutación de ganancia de función en la Drosophila MAP quinasa activa las vías de señalización de la tirosina

D Brunner¹, N Oellers, J Szabad

¹Zoologisches Institut, Universität Zürich, Switzerland.

|March 11, 1994

Resumen

Una mutación en el gen rolled (rlSevenmaker [rlSem]) activa la vía de señalización sevenless (sev) en Drosophila. Esta activación de la MAP quinasa es necesaria y suficiente para múltiples vías receptoras de la tirosina quinasa.

Área de la Ciencia:

Biología del desarrollo Biología del desarrollo.
Genética molecular La genética molecular.
La señalización celular de las células.

Sus antecedentes:

La vía de la tirosina quinasa del receptor sevenless (sev) es crucial para la determinación del destino de las células fotorreceptoras R7 en el ojo de Drosophila.
Comprender la regulación de la señalización de la receptor tirosina quinasa (RTK) es fundamental para la especificación del destino celular durante el desarrollo.

Objetivo del estudio:

Para identificar mutaciones genéticas que causan la activación constitutiva de la vía de señalización sev.
Para investigar el papel de la MAP quinasa en la activación de la vía RTK.

Principales métodos:

Se llevó a cabo un examen genético en Drosophila para encontrar mutaciones de ganancia de función.
Caracterizó una mutación en el gen rodado (rlSevenmaker [rlSem]), que codifica un homólogo de la quinasa MAP.
Analizó los efectos de la mutación rlSem en las vías de homología del receptor del factor de crecimiento sev, torso y epidermal.

Principales resultados:

Se identificó una mutación de ganancia de función, rlSevenmaker (rlSem), en el gen rodado de Drosophila.
La mutación rlSem conduce a la activación constitutiva de la vía de señalización sev.
Esta mutación también activa el torso y las vías de homología del receptor del factor de crecimiento epidérmico, lo que demuestra un papel para la MAP quinasa en múltiples cascadas de señalización de RTK.

Conclusiones:

La mutación rlSem, una sustitución de un solo aminoácido en el dominio de la MAP quinasa, es suficiente para activar múltiples vías de RTK.
La activación de la MAP quinasa es necesaria y suficiente para la activación de estas vías de señalización, destacando su papel central en la señalización del desarrollo.

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