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08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
Published on: May 26, 2017
JNK1: una proteína quinasa estimulada por la luz UV y Ha-Ras que se une y fosforila el dominio de activación c-Jun
1Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biology, University of Massachusetts Medical School, Worcester 01605.
Cell
|March 25, 1994
Resumen
La radiación UV desencadena una respuesta celular que incluye AP-1 y NF-kappa B. Los investigadores identificaron JNK1, una nueva quinasa activada por los rayos UV y las oncoproteínas, que fosforila c-Jun, contribuyendo potencialmente a la promoción del tumor.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología molecular La biología molecular.
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las células de mamíferos exhiben un rápido aumento de la expresión génica tras la exposición a los rayos UV, mediada por AP-1 y NF-kappa B.
- La actividad transcripcional de AP-1 se ve reforzada por el aumento de la fosforilación del dominio de activación c-Jun NH2-terminal.
Objetivo del estudio:
- Para clonar y caracterizar JNK1, una nueva quinasa involucrada en la respuesta UV.
- Investigar el papel de JNK1 en las vías de transducción de señales activadas por la irradiación UV y las oncoproteínas.
Principales métodos:
- Clonación molecular y caracterización del gen JNK1.
- Evaluación de la activación de JNK1 por irradiación UV y Ha-Ras.
- Analizando la unión y fosforilación de JNK1 del dominio de transactivación c-Jun.
Principales resultados:
- JNK1, un pariente de la MAP quinasa, se activa mediante doble fosforilación durante la respuesta UV.
- Ha-Ras activa parcialmente JNK1 y mejora la activación inducida por rayos UV.
- JNK1 fosforila c-Jun en Ser-63 y Ser-73, uniéndose a su dominio de transactivación.
Conclusiones:
- JNK1 es un componente clave de una nueva vía de transducción de señales activada por los rayos UV y las oncoproteínas.
- La activación de JNK1 está implicada en la respuesta celular a la irradiación UV y puede desempeñar un papel en la promoción tumoral.
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