Video Experimental Relacionado

Updated: May 6, 2026

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Characterization of G Protein-coupled Receptors by a Fluorescence-based Calcium Mobilization Assay

Published on: July 28, 2014

La GMP cíclica y la fototransducción de fitocromo mediada por calcio mediado por calcio

C Bowler¹, G Neuhaus, H Yamagata

¹Laboratory of Plant Molecular Biology, Rockefeller University, New York, New York 10021-6399.

|April 8, 1994

Resumen

El GMP cíclico (cGMP) y el calcio son reguladores clave del desarrollo de las plantas. cGMP desencadena la producción de antocianina, mientras que cGMP con calcio promueve el desarrollo de cloroplastos maduros mediante la modulación de la expresión génica.

Área de la Ciencia:

Biología vegetal Biología vegetal
La señalización molecular.
La investigación de la fotosíntesis en la investigación de la fotosíntesis.

Sus antecedentes:

La señalización del fitocromo en las plantas involucra proteínas G, calcio y calmodulina.
La activación de la proteína G ayuda al desarrollo de los cloroplastos y a la síntesis de antocianinas.
El calcio y la calmodulina por sí solos estimulan los cloroplastos inmaduros y no pueden inducir las antocianinas.

Objetivo del estudio:

Investigar el papel del GMP cíclico (cGMP) en la señalización fitocroma.
Determinar los efectos combinados de GMPc y calcio en el desarrollo de los cloroplastos y la producción de antocianinas.
Para dilucidar los mecanismos moleculares por los cuales el cGMP y el calcio regulan estos procesos.

Principales métodos:

Ensayos de una sola célula en un mutante de tomate con deficiencia de fitocromo.
Utilizando genes reporteros (cab-gus, chs-gus, fnr-gus) para monitorear la expresión génica.
Análisis bioquímico del desarrollo de los cloroplastos y la biosíntesis de pigmentos.

Principales resultados:

cGMP solo puede desencadenar la producción de antocianinas.
Una combinación de cGMP y calcio induce a los cloroplastos completamente maduros con una maquinaria fotosintética completa.
Los ensayos del gen Reporter demuestran que el cGMP y el calcio modulan principalmente la expresión génica.

Conclusiones:

cGMP es una molécula de señalización crítica en las vías fitocrómicas, junto con las proteínas G, el calcio y la calmodulina.
La acción sinérgica de cGMP y calcio es esencial para la maduración y función completa de los cloroplastos.
La modulación de la expresión génica es el mecanismo principal a través del cual el cGMP y el calcio ejercen sus efectos en el desarrollo de las plantas.

Más Videos Relacionados

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

Published on: July 16, 2021

High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening

High-Throughput Optical Controlling and Recording Calcium Signal in iPSC-Derived Cardiomyocytes for Toxicity Testing and Phenotypic Drug Screening

Published on: March 31, 2022

Videos de Conceptos Relacionados

Photosystem II

Photosystem II

The multi-protein complex photosystem II (PS II) harvests photons and transfers their energy through its bound pigments to its reaction center, and ultimately to photosystem I (PSI) through the electron transport chain. The pigments responsible for caputirng the light energy in photosystems include chlorophyll a, chlorophyll b, and carotenoids.
The pigment molecules are arranged across two photosystem domains — the antenna complex and the reaction center. The main aim of the pigment...

Photosystem I

Photosystem I

Although structurally similar to photosystem II (PSII), photosystem I (PSI) is has a different electron supplier and electron acceptor.
Both these photosystems work in concert. An excited electron from PSII is relayed to PSI via an electron transport chain in the thylakoid membrane of the chloroplast, which is comprised of the carrier molecule plastoquinone, the dual-protein cytochrome complex, and plastocyanin. As electrons move between PSII and PSI, they lose energy and must be re-energized...

The Calvin Cycle

The Calvin Cycle

OverviewOxygenic photosynthesis plays a central role in the global carbon and oxygen cycles. The carbohydrates produced support nearly all food webs, while the oxygen by‑product enables aerobic life.Light‑dependent and light‑independent reactionsPhotosynthesis occurs in two main stages, each in a different part of the chloroplast: light‑dependent reactions and light‑independent reactions, also called the Calvin‑Benson cycle or simply the Calvin...

Activation and Inactivation of G Proteins

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...

G-Protein Gated Ion Channels

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision. The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...

Oxygenic Photosynthesis

Oxygenic Photosynthesis

Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate...