Video Experimental Relacionado
Updated: Aug 9, 2026
07:49
Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
Published on: August 3, 2018
La clonación de un receptor de TGF beta tipo I que forma un complejo heteromérico con el receptor de TGF beta tipo II
P Franzén1, P ten Dijke, H Ichijo
1Ludwig Institute for Cancer Research, Biomedical Center, Uppsala, Sweden.
Cell
|November 19, 1993
Resumen
Los investigadores identificaron un nuevo receptor de serina / treonina quinasa crucial para la transformación de la señalización del factor de crecimiento beta (TGF-β). Este receptor TGF-β tipo I forma un complejo con el receptor tipo II para mediar las respuestas celulares.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- El factor de crecimiento transformador beta (TGF-β) es un regulador clave de los procesos celulares.
- La señalización del TGF-β involucra receptores específicos de la superficie celular.
- Se está investigando la composición precisa de los complejos de receptores de TGF-β.
Objetivo del estudio:
- Clonar y caracterizar un nuevo receptor de serina/treonina quinasa involucrado en la señalización del TGF-β.
- Para aclarar el papel de este receptor en la transducción de señales de TGF-β.
- Determinar si este receptor forma un complejo con el conocido receptor TGF-β de tipo II.
Principales métodos:
- clonación de ADNc y expresión de un nuevo receptor de serina/treonina quinasa.
- Ensayos de unión de ligandos utilizando TGF-β1.1, marcado radiactivamente.
- Estudios de enlace cruzado para identificar complejos de receptores.
- Inmunoprecipitación utilizando anticuerpos contra el receptor clonado.
- Análisis funcionales que evalúan la expresión génica inducida por TGF-β.
Principales resultados:
- Se aisló un cDNA que codifica un receptor de serina/treonina quinasa de 53 kDa, estructuralmente similar al receptor de TGF-β tipo II.
- El receptor clonado exhibió propiedades bioquímicas consistentes con un receptor de TGF-β tipo I.
- Los estudios vinculantes revelaron una característica compleja de 70 kDa de un receptor TGF-β de tipo I.
- La inmunoprecipitación identificó complejos de receptores de 70 kDa (tipo I) y 94 kDa (tipo II).
- Restauración de la producción del inhibidor 1 del activador del plasminógeno inducido por TGF-β en las células deficientes tras la transfección.
Conclusiones:
- Un nuevo receptor de serina/treonina quinasa funciona como el receptor de TGF-β tipo I.
- La transducción de la señal TGF-β implica la formación de un complejo heteromérico entre los receptores tipo I y tipo II.
- Esta formación de complejos heteroméricos es esencial para mediar los efectos biológicos del TGF-β.
Más Videos Relacionados
06:54Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Published on: October 27, 2020
07:05TGF-β-mediated Endothelial to Mesenchymal Transition (EndMT) and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing
Published on: February 26, 2021
Videos de Conceptos Relacionados
02:30Metastasis
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
01:15Transcytosis of IgG
Transcytosis is the process in which molecules are internalized by endocytosis, transported across the cell, and released through exocytosis from the opposite end of the cell. Molecules such as insulin, immunoglobulins, and certain nutrients are transferred through the recycling endosomes by recycling and transcytosis.
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
IgG molecules from a mother undergo transcytosis starting around 13 weeks of gestation. The amount of IgG transferred and entering the fetal blood circulation increases with...
01:09Introduction to Fibroblasts
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
01:26Receptor Tyrosine Kinases
Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
01:15Activation of Integrins
Integrins bind ligands and transmit information from outside the cell to inside or vice-versa through an "outside-in signaling" or "inside-out signaling."
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.
In "outside-in signaling," external factors in the extracellular space bind to exposed ligand binding sites on integrins. This causes the inactive protein to undergo a conformational change to become active. Integrins are often clustered on the cell membrane. Repetitive and regularly spaced ligand binding events provide an effective stimulus.