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El colapso del cono de crecimiento neuronal y la inhibición de la acilación de proteínas grasas por el óxido nítrico

D T Hess¹, S I Patterson, D S Smith

¹Department of Cell Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

|December 9, 1993

Resumen

El óxido nítrico (NO) inhibe el crecimiento de neuritas en neuronas en desarrollo al afectar la acilación de proteínas grasas. Esto sugiere que el NO regula el desarrollo neuronal y la remodelación.

Área de la Ciencia:

La neurociencia es la neurociencia.
Biología celular Biología celular.
Biología del desarrollo Biología del desarrollo.

Sus antecedentes:

El óxido nítrico (NO) actúa como molécula de señalización en el sistema nervioso central maduro.
Los modelos teóricos sugieren que el NO también puede regular el desarrollo neuronal.
El papel del NO en el desarrollo neuronal no se entiende completamente.

Objetivo del estudio:

Investigar el papel del óxido nítrico en el desarrollo de las conexiones neuronales.
Determinar el efecto del óxido nítrico en el crecimiento de neuritas en neuronas en desarrollo.

Principales métodos:

Cultivo in vitro de neuronas ganglionares de la raíz dorsal de la rata.
Exposición al gas de óxido nítrico.
Análisis del crecimiento de las neuritas.
Evaluación de la acilación proteica de las grasas.

Principales resultados:

El óxido nítrico inhibió rápida y reversiblemente el crecimiento de neuritas en neuronas cultivadas.
La exposición al óxido nítrico redujo la acilación de las grasas de cadena larga ligadas al tioester de las proteínas neuronales.
La inhibición de la acilación de grasas puede ocurrir a través de la modificación de los tiolos de cisteína.

Conclusiones:

El óxido nítrico juega un papel en la regulación del crecimiento de las neuritas durante el desarrollo neuronal.
El NO puede modular la acilación de proteínas grasas en los conos de crecimiento neuronal, influyendo en la remodelación del proceso.

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