Video Experimental Relacionado
Updated: Jun 23, 2026
10:30
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
Published on: January 12, 2014
Los genes de la semaforina codifican una familia de moléculas guía transmembrana y secreta del cono de crecimiento
A L Kolodkin1, D J Matthes, C S Goodman
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Cell
|December 31, 1993
Resumen
Este estudio identifica y caracteriza los genes semaphorin en Drosophila y humanos, revelando su papel crucial en el desarrollo neuronal y la supervivencia adulta. Semaphorin I guía los conos de crecimiento sensorial en los límites epiteliales.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La neurociencia es la neurociencia.
- Genética molecular genética molecular.
Sus antecedentes:
- Semaphorin I (Sema I) guía los conos de crecimiento sensorial en los límites epiteliales en las yemas de las extremidades de los saltamontes.
- Las semaforinas son una familia conservada de proteínas involucradas en la guía celular.
Objetivo del estudio:
- Clonar y caracterizar los genes de la semaforina en Drosophila y en humanos.
- Para investigar la función de los genes de semaphorin en el desarrollo neuronal y el comportamiento.
Principales métodos:
- La clonación de genes y la caracterización de genes de semaphorin en Drosophila y humanos.
- Identificación de las secuencias de semaforinas virales relacionadas.
- Análisis de expresión de genes de Drosophila semaphorin en neuronas y músculos.
- Análisis genético en Drosophila para determinar la esencialidad genética.
Principales resultados:
- Se clonaron y caracterizaron dos genes de semaphorina de Drosophila (D-Sema I y D-Sema II) y un gen de semaphorina humana (Sema III).
- D-Sema I es una proteína transmembrana, mientras que D-Sema II y el Sema III humano son proteínas secretadas.
- D-Sema I y D-Sema II se expresan en subconjuntos de neuronas y músculos.
- Drosophila sema II es esencial para el comportamiento y la supervivencia de los adultos.
Conclusiones:
- Las semaforinas se conservan en todas las especies y juegan un papel crítico en el desarrollo neuronal.
- La Drosophila semaphorin II es esencial para el correcto funcionamiento del sistema nervioso, afectando el comportamiento y la supervivencia.
Videos de Conceptos Relacionados
03:14Notch Signaling Pathway
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not until 1985...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:30Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
01:24Small GTPases - Ras and Rho
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...