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Un complejo de múltiples subunidades asociado con la ARN polimerasa II CTD y la proteína de unión TATA en levaduras
C M Thompson1, A J Koleske, D M Chao
1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Massachusetts 02142.
Cell
|July 2, 1993
Resumen
Los investigadores identificaron nuevas proteínas SRB (SRB2, SRB4, SRB5, SRB6) que interactúan con el dominio carboxiterminal (CTD) de la ARN polimerasa II. Estas proteínas SRB, junto con la proteína de unión TATA (TBP), forman un complejo esencial para la iniciación eficiente de la transcripción génica.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- La bioquímica es la bioquímica.
Sus antecedentes:
- El dominio carboxiterminal (CTD) de la ARN polimerasa II juega un papel crucial en la transcripción.
- Comprender las interacciones proteicas que involucran a la CTD es clave para descifrar la regulación de la transcripción.
Objetivo del estudio:
- Para identificar y caracterizar los factores que interactúan con la ARN polimerasa II CTD.
- Para aclarar la composición y función del complejo de iniciación de la transcripción.
Principales métodos:
- Cribado genético para los supresores extragénicos de las mutaciones de truncamiento de CTD en S. cerevisiae.
- Aislamiento bioquímico y caracterización de complejos proteicos.
- Pruebas de transcripción in vitro y ensayos de compromiso de la plantilla.
Principales resultados:
- Identificación de cuatro genes SRB (SRB2, SRB4, SRB5, SRB6) involucrados en la función de la DTC.
- Descubrimiento de un gran complejo multisubunitaria que contiene proteínas SRB y proteína de unión a TATA (TBP).
- Demostrar que este complejo SRB-TBP se une al CTD de la ARN polimerasa II y es necesario para una iniciación eficiente de la transcripción.
Conclusiones:
- El complejo SRB-TBP es un componente integral de la maquinaria de iniciación de la transcripción.
- SRB2 y SRB5 son esenciales para la formación de un complejo de preiniciación funcional.
- Estos hallazgos proporcionan información crítica sobre la regulación de la transcripción génica por la ARN polimerasa II.
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