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Los dominios SH3 dirigen la localización celular de las moléculas de señalización
D Bar-Sagi1, D Rotin, A Batzer
1Cold Spring Harbor Laboratory, New York 11724.
Cell
|July 16, 1993
Resumen
El dominio SH3, no el dominio SH2, dirige las proteínas de señalización como la fosfolipasa C-gamma (PLC-gamma) y GRB2 a ubicaciones celulares específicas, como el citoesqueleto de actina y los volantes de la membrana.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- Las vías de señalización dependen de la localización de proteínas para su función.
- La fosfolipasa C-gamma (PLC-gamma) y el GRB2 son adaptadores de señalización clave.
- Los roles de los dominios SH2 y SH3 en la localización de proteínas no se comprenden completamente.
Objetivo del estudio:
- Para investigar la distribución celular de los dominios PLC-gamma y GRB2.
- Para determinar los dominios específicos responsables de la orientación subcelular.
Principales métodos:
- Microinyección de proteínas truncadas PLC-gamma y GRB2 en fibroblastos de embriones de ratas.
- Microscopía de inmunofluorescencia para visualizar la localización de proteínas.
- Análisis de proteínas con mutaciones de pérdida de función en los dominios SH2 o SH3.
Principales resultados:
- Los dominios PLC-gamma SH2/SH3 y SH3 están localizados en el citoesqueleto de actina.
- Los dominios PLC-gamma SH2 mostraron una distribución difusa en el citoplasma.
- GRB2 localizado en las arrugas de la membrana, independiente de las interacciones del dominio SH2.
- GRB2 con mutaciones SH3 exhibió una distribución difusa en el citoplasma.
Conclusiones:
- Los dominios SH3 son críticos para dirigir las moléculas de señalización a ubicaciones subcelulares específicas.
- Los dominios SH2 no son suficientes para la localización en el citoesqueleto de actina.
- La localización de GRB2 a las arrugas de la membrana está mediada por su dominio SH3.
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