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La remodelación de la actina inducida por el factor de crecimiento epidérmico está regulada por los productos de
M P Peppelenbosch1, L G Tertoolen, W J Hage
1Hubrecht Laboratory, Netherlands Institute for Developmental Biology, Utrecht.
Cell
|August 13, 1993
Resumen
El factor de crecimiento epidérmico (EGF) desencadena cambios en la forma celular y la reorganización de la actina a través de metabolitos del ácido araquidónico. Las vías de lipoxigenasa y ciclooxigenasa median eventos de actina distintos, revelando un nuevo mecanismo de señalización.
Área de la Ciencia:
- Biología celular Biología celular.
- La bioquímica es la bioquímica.
- La señalización molecular.
Sus antecedentes:
- El factor de crecimiento epidérmico (EGF) induce cambios significativos en la morfología celular, incluida la polimerización de actina y la descomposición de la fibra por estrés.
- Todavía no están claros los mecanismos moleculares precisos que vinculan la actividad de la tirosina quinasa del receptor EGF con la reorganización citosquelética.
- Recientemente se han identificado metabolitos del ácido araquidónico como participantes en la transducción de señales del FEA.
Objetivo del estudio:
- Aclarar el papel de los metabolitos del ácido araquidónico en la mediación de las alteraciones citoesqueléticas inducidas por el FEAG.
- Diferenciar las contribuciones de las vías lipoxigenasa y ciclooxigenasa en la señalización del FEAG.
- Comprender el mecanismo detrás del redondeo celular provocado por el FEAG.
Principales métodos:
- Investigó la polimerización de actina inducida por el FEAG y la descomposición de la fibra por estrés en células A431, células HeLa y fibroblastos de rata-1.
- Evaluó la participación de las vías de lipoxigenasa y ciclooxigenasa utilizando inhibidores específicos o analizando la producción de metabolitos.
- Cambios morfológicos celulares dependientes del FEAG monitoreados, específicamente el redondeo celular.
Principales resultados:
- La polimerización de la actina cortical inducida por el FEG está mediada por el metabolismo de la lipoxigenasa.
- La descomposición de las fibras de estrés inducida por el FEAG está mediada por los metabolitos de la ciclooxigenasa.
- El redondeo celular provocado por el FEA en las células A431 depende del metabolismo del ácido araquidónico.
Conclusiones:
- Los leucotrienos y las prostaglandinas actúan conjuntamente como segundos mensajeros en la transducción de señales del FEAG.
- Estos metabolitos orquestan los efectos morfológicos y la reorganización de la actina.
- Este estudio revela un nuevo mecanismo para los cambios citoesqueléticos inducidos por el factor de crecimiento.
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