Video Experimental Relacionado
Updated: Aug 10, 2026
10:58
Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
BUD2 codifica una proteína activadora de la GTPasa para Bud1/Rsr1 necesaria para la correcta selección del sitio de
H O Park1, J Chant, I Herskowitz
1Department of Biochemistry & Biophysics, University of California, San Francisco 94143-0448.
Nature
|September 16, 1993
Resumen
La proteína Bud2 actúa como una proteína activadora de la GTPasa (GAP) para la proteína Bud1 en el brote de la levadura. Este descubrimiento aclara los mecanismos moleculares que rigen la selección del sitio del brote en Saccharomyces cerevisiae.
Área de la Ciencia:
- Biología celular Biología celular.
- Genética molecular genética molecular.
- Genética de la levadura genética de la levadura.
Sus antecedentes:
- La levadura de brote Saccharomyces cerevisiae exhibe una selección axial o bipolar del sitio del brote.
- La selección del sitio del brote es crucial para la polaridad celular y la organización citoesquelética.
- Cinco genes, incluidos BUD1/RSR1, BUD2, BUD3, BUD4 y BUD5, regulan la selección del sitio del brote.
Objetivo del estudio:
- Investigar la función de la proteína Bud2 en el contexto de la selección del sitio del brote.
- Para determinar la actividad bioquímica de la proteína Bud2 en relación con la proteína Bud1.
Principales métodos:
- Pruebas bioquímicas utilizando la proteína Bud2 purificada.
- Análisis de los patrones de brote en las levaduras mutantes (deleción cromosómica y sobreexpresión).
Principales resultados:
- La proteína Bud2 funciona como una proteína activadora de la GTPasa (GAP) para la proteína Bud1.
- La proteína Bud2 purificada estimula la hidrólisis de GTP por la proteína Bud1.
- La deleción o sobreexpresión de BUD2 conduce a una selección aleatoria del sitio del brote en las células de levadura.
Conclusiones:
- Bud2p actúa como una proteína activadora de la GTPasa (GAP) para Bud1p, un homólogo de Ras.
- Esta interacción es crítica para la selección adecuada del sitio del brote en Saccharomyces cerevisiae.
- El gen BUD2 juega un papel clave en la regulación de la polaridad celular durante la germinación.
Videos de Conceptos Relacionados
01:28Yeast Signaling
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
01:33Coat Assembly and GTPases
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
01:14Rab Proteins
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
01:25Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
01:24Small GTPases - Ras and Rho
Ras and Rho are small monomeric GTPases that act downstream of receptor tyrosine kinase (RTK) and regulate various cellular processes. These GTPases switch between active and inactive states by binding to guanine nucleotides.
Three regulatory proteins control their activity:
Three regulatory proteins control their activity:
01:17Gene Regulation During Sporulation
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...