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La proteína accesoria HCF del VP16 es una familia de polipéptidos procesados a partir de una gran proteína precursora
A C Wilson1, K LaMarco, M G Peterson
1Cold Spring Harbor Laboratory, New York 11724.
Cell
|July 16, 1993
Resumen
La proteína transactivadora del virus del herpes simple (HSV) VP16 requiere el factor de la célula huésped (HCF) para la activación transcripcional. Este estudio revela que el HCF existe como múltiples polipéptidos derivados de un solo gen a través del procesamiento.
Área de la Ciencia:
- Virología Virología.
- Biología Molecular Biología Molecular
- Regulación genética Reglamento genético.
Sus antecedentes:
- La transcripción genética temprana inmediata del virus del herpes simple (HSV) está regulada por el transactivador VP16.
- La actividad de VP16 depende de la interacción con el factor celular huésped (HCF) y Oct-1.
Objetivo del estudio:
- Investigar la naturaleza molecular y el origen del factor celular huésped (HCF).
- Para aclarar la relación entre las diferentes formas de polipéptido HCF.
Principales métodos:
- Análisis de la composición del polipéptido HCF en células infectadas.
- clonación de ADNc y expresión de HCF en células humanas.
- Identificación de un nuevo motivo de repetición de 26 aminoácidos dentro de HCF.
Principales resultados:
- El factor de célula huésped (HCF) consiste en múltiples polipéptidos relacionados (110-300 kd) codificados por un solo gen.
- Un nuevo motivo de repetición de 26 aminoácidos está presente ocho veces en HCF.
- La expresión de un marco de lectura abierto HCF grande produce polipéptidos HCF grandes y pequeños.
Conclusiones:
- Los polipéptidos HCF más pequeños se derivan de la forma 300 kd más grande a través del procesamiento.
- Los hallazgos proporcionan información sobre la complejidad estructural y la regulación post-traducional del HCF.
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