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Analysis of Cell Cycle Position in Mammalian Cells
Published on: January 21, 2012
Análisis funcional de la caja P, un dominio en la ciclina B requerido para la activación de Cdc25
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|October 8, 1993
Resumen
El dominio de la caja P de la ciclina B es crucial para la activación de la fosfatasa cdc25. Este hallazgo apoya un mecanismo de dos pasos para la activación del complejo ciclina B-cdc2 durante el ciclo celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- La bioquímica es la bioquímica.
Sus antecedentes:
- Los complejos ciclina B-cdc2 regulan la progresión del ciclo celular.
- Las fosforilaciones inhibidoras mantienen la inactivación de cdc2 hasta finales de G2.
- La fosfatasa cdc25 activa los complejos ciclina B-cdc2 para la entrada en la fase M.
Objetivo del estudio:
- Para investigar el papel de la caja ciclina B P en la activación de cdc25.
- Para aclarar el mecanismo de la activación de la ciclina B-cdc2 dependiente de cdc25.
Principales métodos:
- Ensayos de unión in vitro de las proteínas mutantes de ciclina B con cdc2.
- Análisis de los fenotipos de detención del ciclo celular.
- Evaluación de la actividad de la fosfatasa cdc25 en presencia de ciclina B. tipo silvestre y mutante.
Principales resultados:
- Las proteínas mutantes de ciclina B con cajas P alteradas se unen a cdc2 pero no logran activar cdc25.
- Las células que expresan proteínas mutantes de ciclina B muestran una detención del ciclo celular en G2.
- La actividad de la fosfatasa cdc25 depende de la caja ciclina B P, con una regulación distinta de las formas de interfase y fase M.
Conclusiones:
- El dominio de la caja P de la ciclina B es esencial para la activación de cdc25.
- Se apoya un mecanismo de dos pasos para la activación dependiente de cdc25 de los complejos ciclina B-cdc2.
- Esta investigación aclara un paso regulatorio crítico en el control del ciclo celular.
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