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Tissue Determination Using the Animal Cap Transplant (ACT) Assay in Xenopus laevis
Published on: May 17, 2010
La proteína noggin secretada imita el organizador de Spemann en la dorsalización del mesodermo de Xenopus
1Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Nature
|February 11, 1993
Resumen
La proteína noggin, expresada en el organizador de Spemann, actúa como una señal de dorsalización durante la gastrulación. Induce la formación de músculo en los tejidos ventrales, a diferencia de la activina, lo que sugiere que el noggin es la señal dorsalizante clave.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La embriogénesis es la embriogénesis.
- La señalización molecular.
Sus antecedentes:
- Durante la gastrulación, una señal de dorsalización del organizador de Spemann especifica los tejidos mesodérmicos laterales hacia los destinos dorsales (músculo, corazón, pronefros) en lugar de los ventrales (sangre, mesénquima).
- Los inductores conocidos del mesodermo como la activina y el factor de crecimiento de los fibroblastos no pueden replicar este efecto dorsalizante.
Objetivo del estudio:
- Para investigar si el gen noggin, expresado en el organizador, funciona como la señal de dorsalización.
- Para determinar si la proteína noggin puede inducir destinos mesodérmicos dorsales.
Principales métodos:
- Adición de proteína noggin soluble a las zonas marginales ventrales durante la gastrulación.
- Evaluación de la especificación del tejido mesodérmico en respuesta a noggin.
- Rescate parcial del patrón dorsal en embriones ventralizados mediante inyección de plásmido para expresar la cabeza.
Principales resultados:
- La proteína noggin soluble indujo la formación muscular en las zonas marginales ventrales.
- Activin no indujo la formación muscular bajo las mismas condiciones.
- La expresión noggin a través de la inyección de plásmido rescató parcialmente el patrón dorsal en embriones ventralizados.
Conclusiones:
- Los hallazgos sugieren fuertemente que el producto del gen noggin es la señal de dorsalización que se origina en el organizador de Spemann.
- Noggin juega un papel crucial en el establecimiento de patrones dorsal-ventral durante el desarrollo embrionario.
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