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Utilizing the Antigen Capsid-Incorporation Strategy for the Development of Adenovirus Serotype 5-Vectored Vaccine Approaches

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Las integrinas alfa v beta 3 y alfa v beta 5 promueven la internalización del adenovirus, pero no la fijación del

T J Wickham¹, P Mathias, D A Cheresh

¹Scripps Research Institute, Department of Immunology, La Jolla, California 92037.

|April 23, 1993

Resumen

El adenovirus utiliza proteínas virales distintas para la adherencia celular y la internalización. Las integrinas alfa v beta 3 y alfa v beta 5 son cruciales para la captación del adenovirus, no para la fijación inicial.

Área de la Ciencia:

Virología Virología.
Biología celular Biología celular.
Biología Molecular Biología Molecular

Sus antecedentes:

La infección por adenovirus implica la fijación viral y la entrada en las células huésped.
El virus utiliza una proteína de fibra para la fijación y una base de pentón con secuencias Arg-Gly-Asp, lo que sugiere la participación de la integrina.

Objetivo del estudio:

Aclarar las funciones específicas de las integrinas en la internalización del adenovirus.
Investigar el mecanismo por el cual las integrinas median la infección por adenovirus.

Principales métodos:

Utilizó anticuerpos contra los receptores de integrina (alfa v beta 3, alfa v beta 5) y la base pentónica soluble.
Estudió la internalización del adenovirus en células con y sin expresión de alfa v integrina.
Transfección de ADNc empleada para restaurar la expresión de integrina en células deficientes.

Principales resultados:

Los anticuerpos y la base pentónica soluble inhibieron la internalización del adenovirus, pero no su adherencia.
Las células que carecen de alfa v integrinas se unen al adenovirus pero no internalizan el virus.
La restauración de la expresión de alfa v integrina permitió la internalización del adenovirus y la infección.

Conclusiones:

El apego y la internalización del adenovirus son procesos distintos y cooperativos.
Las proteínas de la fibra median el apego, mientras que las alfa v integrinas (alfa v beta 3, alfa v beta 5) median la internalización.

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