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FAR1 enlaza la vía de transducción de la señal con la maquinaria del ciclo celular en la levadura
M Peter1, A Gartner, J Horecka
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Cell
|May 21, 1993
Resumen
El factor alfa desencadena la detención del ciclo celular de la levadura a través de la cinasa FUS3 y la proteína FAR1. La fosforilación de FAR1 por FUS3 regula su interacción con CDC28-CLN2, vinculando la señalización al control del ciclo celular.
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Genética de la levadura Genética de la levadura
Sus antecedentes:
- La feromona del factor alfa en la levadura (Saccharomyces cerevisiae) detiene las células en la fase G1.
- Esta detención es crucial para el apareamiento e implica la inhibición de las ciclinas G1.
- FUS3 (MAP quinasa) y FAR1 están implicados en este mecanismo de detención del ciclo celular.
Objetivo del estudio:
- Para dilucidar el mecanismo molecular por el cual FAR1 contribuye a la detención del ciclo celular G1.
- Para investigar la relación entre FUS3, FAR1 y el complejo de quinasa dependiente de ciclina CDC28-CLN2.
Principales métodos:
- Ensayos in vitro de la quinasa para determinar la fosforilación FUS3 de FAR1.1.
- Análisis de la formación del complejo FAR1-CDC28-CLN2.
- Estudios de fosforilación in vivo.
- El análisis mutacional de FAR1.1.
Principales resultados:
- FAR1 es un sustrato directo de la FUS3 quinasa.
- La fosforilación mediada por FUS3 de FAR1 regula su asociación con la cinasa CDC28-CLN2.
- FAR1 también es fosforilado por CDC28-CLN2 de una manera dependiente del ciclo celular.
- La capacidad de FAR1 para unirse a CDC28-CLN2 se correlaciona con su función de detención del ciclo celular.
Conclusiones:
- FAR1 actúa como un enlace crítico entre la vía de señalización de feromonas y la maquinaria del ciclo celular central.
- Los eventos de fosforilación que involucran a FUS3 y CDC28-CLN2 son pasos reguladores clave en la función de FAR1.
- Estos hallazgos proporcionan una base molecular de cómo las feromonas de apareamiento controlan el ciclo celular de la levadura.
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