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Migración no lineal de ribosomas en el virus del mosaico de la coliflor 35S ARN
J Fütterer1, Z Kiss-László, T Hohn
1Friedrich Miescher-Institute, Basel, Switzerland.
Cell
|May 21, 1993
Resumen
Las secuencias de líder de ARN 35S del virus del mosaico de la coliflor pueden inhibir la traducción, pero elementos específicos permiten la expresión génica aguas abajo en las células vegetales. Este proceso implica eludir las regiones internas a través de complejos de escaneo, incluso entre moléculas de ARN separadas.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Virología Vegetal Virología Vegetal
- Regulación de la expresión génica Regulación de la expresión génica
Sus antecedentes:
- El virus del mosaico de la coliflor (CaMV) 35S ARN presenta una secuencia líder de 600 nucleótidos.
- Este líder contiene pequeños marcos de lectura abiertos (sORF) que pueden reprimir la traducción de las regiones de codificación aguas abajo.
Objetivo del estudio:
- Investigar los mecanismos regulatorios que rigen la traducción de las regiones de codificación aguas abajo dentro del líder de ARN CaMV 35S.
- Para identificar los elementos específicos de ARN esenciales o prescindibles para el inicio de la traducción en ciertas células vegetales.
Principales métodos:
- Análisis de las secuencias líderes de ARN CaMV 35S y su impacto en la traducción aguas abajo.
- Mutagenesis de la secuencia líder, incluida la inserción de estructuras de bucles del tallo y regiones de codificación.
- Investigando la traducción en tipos específicos de células vegetales.
- Examinando la derivación de acción trans entre moléculas de ARN separadas.
Principales resultados:
- La traducción de las regiones de codificación aguas abajo está permitida en células vegetales específicas, dependientes del extremo 5' del ARN y otros elementos líderes.
- La parte central del líder es prescindible o puede acomodar inserciones sin abolir la traducción.
- Un fenómeno llamado "shunting" permite que el complejo de exploración eluda las regiones inhibidoras.
- También se demostró que la derivación se produce en trans entre moléculas de ARN distintas.
Conclusiones:
- El líder de ARN CaMV 35S emplea un mecanismo de derivación para superar la represión traslacional por parte de las sORF.
- Esta derivación permite una expresión eficiente de los genes aguas abajo, regulados por características específicas de ARN.
- La capacidad de derivación es un mecanismo flexible que puede operar tanto cis como trans.
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