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Una subclase de proteínas bHLH requeridas para la morfogénesis cardíaca
D Srivastava1, P Cserjesi, E N Olson
1Department of Biochemistry and Molecular Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030, USA.
Science (New York, N.Y.)
|December 22, 1995
Resumen
Dos factores de transcripción específicos del músculo, dHAND y eHAND, son cruciales para el desarrollo del corazón de los vertebrados. Juntos, regulan la formación del tubo cardíaco, lo que sugiere roles redundantes en la morfogénesis del corazón embrionario.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- Genética molecular genética molecular.
Sus antecedentes:
- El desarrollo del músculo esquelético depende de los factores de transcripción básicos hélice-bucle-hélice (bHLH).
- Los roles de los genes bHLH específicos en el desarrollo temprano del corazón no se comprenden completamente.
Objetivo del estudio:
- Para investigar la función de los genes dHAND y eHAND en la embriogénesis de los vertebrados.
- Para determinar la participación de dHAND y eHAND en el desarrollo del corazón.
Principales métodos:
- Aislamiento y caracterización de los genes dHAND y eHAND.
- Análisis de la expresión en embriones de pollo y ratón.
- Tratamiento de oligonucleótidos antisense de embriones de pollo en estadio 8.
Principales resultados:
- dHAND y eHAND se expresan en el corazón primordial en desarrollo y en el corazón tubular.
- Los oligonucleótidos antisense para dHAND o eHAND por sí solos no afectaron el desarrollo.
- Los oligonucleótidos antisense combinados dHAND y eHAND detuvieron el desarrollo embrionario en la etapa del tubo cardíaco en bucle.
Conclusiones:
- dHAND y eHAND desempeñan funciones esenciales y potencialmente redundantes en la regulación del desarrollo del corazón de los vertebrados.
- Estos genes son críticos para los eventos morfogenéticos que conducen a la formación del tubo cardíaco.
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