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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
Published on: February 20, 2017
Interacción de Cdc2 y Cdc18 con una proteína similar a la ORC2 de la levadura de fisión
J Leatherwood1, A Lopez-Girona, P Russell
1Department of Molecular Biology, Scripps Research Institute, La Jolla, California 92037, USA.
Nature
|January 25, 1996
Resumen
La quinasa Cdc2 de la levadura de fisión interactúa con Orp2, una proteína esencial para el inicio de la replicación del ADN. Esta interacción sugiere funciones conservadas del complejo de reconocimiento del origen eucariótico (CRO) y Cdc2 .
Área de la Ciencia:
- Biología celular Biología celular.
- Biología Molecular Biología Molecular
- Genética La genética.
Sus antecedentes:
- La quinasa Cdc2 regula la replicación del ADN en la levadura de fisión, controlando la entrada en la fase S y evitando la reiniciación.
- El complejo de reconocimiento de origen (ORC) une los orígenes cromosómicos, iniciando la replicación del ADN.
- Orp2 es una proteína similar a Orc2, una subunidad de Saccharomyces cerevisiae ORC.
Objetivo del estudio:
- Para investigar la interacción entre Cdc2 quinasa y Orp2 en la levadura de fisión.
- Para aclarar el papel de Orp2 en la replicación del ADN y el control del ciclo celular.
- Proporcionar evidencia de las funciones ORC conservadas en eucariotas y el papel de Cdc2 en la iniciación de la replicación.
Principales métodos:
- Estudios de interacción de proteínas (por ejemplo, levadura de dos híbridos, co-inmunoprecipitación).
- Análisis de la replicación del ADN y la progresión del ciclo celular en cepas de levadura de fisión de tipo salvaje y mutante.
- Investigación de la progresión mitótica y la señalización de puntos de control.
Principales resultados:
- La quinasa Cdc2 interactúa con Orp2, una proteína homóloga a Orc2.
- La levadura de fisión Orp2 es esencial para la replicación del ADN e interactúa con Cdc18, un activador de la replicación.
- Las células que carecen de Orp2 muestran mitosis aberrante, lo que sugiere un papel en la señalización de puntos de control.
Conclusiones:
- La levadura de fisión Orp2 es crucial para el inicio de la replicación del ADN y la progresión del ciclo celular.
- Los hallazgos apoyan las funciones conservadas del complejo de reconocimiento de origen (CRO) en todos los eucariotas.
- La quinasa Cdc2 probablemente controla el inicio de la replicación del ADN actuando directamente en los orígenes cromosómicos.
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