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La holoenzima ARN polimerasa II contiene reguladores SWI / SNF involucrados en la remodelación de la cromatina
C J Wilson1, D M Chao, A N Imbalzano
1Whitehead Institute for Biomedical Research Nine Cambridge Center, Massachusetts 02142, USA.
Cell
|January 26, 1996
Resumen
Las proteínas SWI / SNF son parte integral de la holoenzima ARN polimerasa II de la levadura, que actúa como reguladores genéticos globales. Estos componentes remodelan la cromatina, facilitando la unión del complejo de iniciación de la transcripción en los promotores.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Genética La genética.
- La bioquímica es la bioquímica.
Sus antecedentes:
- La holoenzima ARN polimerasa II es un complejo crucial para la transcripción génica.
- Los factores generales de transcripción y las proteínas SRB son componentes conocidos de esta holoenzima.
- Los productos genéticos SWI y SNF son reguladores genéticos globales establecidos involucrados en la remodelación de la cromatina.
Objetivo del estudio:
- Para investigar el papel de los productos de los genes SWI y SNF en la holoenzima ARN polimerasa II de la levadura.
- Para determinar si las proteínas SWI/SNF son componentes integrales de la holoenzima.
- Para entender cómo las proteínas SWI/SNF contribuyen a la función de la holoenzima.
Principales métodos:
- Análisis bioquímico de la holoenzima ARN polimerasa II de la levadura.
- Identificación de los componentes de las proteínas dentro del complejo holoenzimático.
- Pruebas para evaluar la contribución funcional de las proteínas SWI/SNF.
Principales resultados:
- Los productos de los genes SWI y SNF fueron identificados como componentes integrales de la holoenzima ARN polimerasa II de la levadura.
- Estas proteínas SWI / SNF son parte del complejo SRB (mediador) asociado con el dominio de repetición C-terminal de la ARN polimerasa II.
- Los componentes SWI/SNF permiten a la holoenzima interrumpir el ADN nucleosómico.
Conclusiones:
- Las proteínas SWI/SNF son componentes esenciales de la holoenzima ARN polimerasa II de la levadura, extendiendo sus funciones conocidas más allá de la regulación genética global.
- La inclusión de proteínas SWI/SNF le otorga a la holoenzima la capacidad de remodelar la estructura de la cromatina.
- Esta capacidad de remodelación de la cromatina facilita el ensamblaje estable del complejo de iniciación de la transcripción en las regiones promotoras, mejorando la eficiencia transcripcional.
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