Video Experimental Relacionado
Updated: May 13, 2026
10:07
Identification of Post-translational Modifications of Plant Protein Complexes
Published on: February 22, 2014
El locus de resistencia a la enfermedad Cf-2 del tomate comprende dos genes funcionales que codifican las proteínas
M S Dixon1, D A Jones, J S Keddie
1Sainsbury Laboratory, John Innes Centre, Norwich, United Kingdom.
Cell
|February 9, 1996
Resumen
Los genes de resistencia de las plantas como Cf-2 son cruciales para la defensa del patógeno. Este estudio aisló los genes Cf-2 del tomate, revelando su papel en el reconocimiento de genes específicos de avirulencia (Avr) de C. fulvum, mejorando la inmunidad de la planta.
Área de la Ciencia:
- Biología molecular vegetal Biología molecular vegetal
- Interacciones entre plantas y patógenos.
- Genética La genética.
Sus antecedentes:
- La resistencia a las enfermedades de las plantas a menudo está mediada por genes de resistencia dominantes.
- Se supone que los productos de estos genes detectan proteínas de avirulencia (Avr) codificadas por patógenos.
- El patógeno del moho de la hoja de tomate, *C. fulvum*, sirve como modelo para estudiar el reconocimiento de patógenos vegetales.
Objetivo del estudio:
- Para aislar y caracterizar el locus de resistencia del tomate Cf-2.
- Comprender la base molecular de la resistencia mediada por Cf-2 a *C. fulvum*.
- Investigar las características estructurales de las proteínas Cf-2 y su papel potencial en la señalización de defensa de las plantas.
Principales métodos:
- Se empleó la clonación posicional para aislar el locus Cf-2 del tomate.
- Se realizó un análisis de secuencia en los genes Cf-2 identificados.
- Se realizó un análisis comparativo con otros genes de resistencia conocidos, como Cf-9.
Principales resultados:
- El locus Cf-2 contiene dos genes muy similares, cada uno confiere resistencia a cepas específicas de *C. fulvum* que expresan el gen Avr2 correspondiente.
- Los genes Cf-2 codifican proteínas con 38 motivos de repetición ricos en leucina (LRR), 20 de los cuales exhiben repeticiones alternas conservadas.
- Las regiones C-terminales de las proteínas Cf-2 muestran homología con el producto genético Cf-9.
Conclusiones:
- Los genes Cf-2 identificados son esenciales para reconocer los factores de avirulencia de *C. fulvum*.
- Es probable que las características estructurales de las proteínas Cf-2, en particular los motivos LRR, estén involucradas en el reconocimiento de patógenos.
- La región C-terminal conservada puede interactuar con los componentes de señalización aguas abajo para activar los mecanismos de defensa de la planta.
Más Videos Relacionados
09:05Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
Published on: March 11, 2020
06:41High-Throughput Identification of Resistance to Pseudomonas syringae pv. Tomato in Tomato using Seedling Flood Assay
Published on: March 10, 2020
Videos de Conceptos Relacionados
01:18Dihybrid Crosses
Overview
01:39Epistasis
In addition to multiple alleles at the same locus influencing traits, numerous genes or alleles at different locations may interact and influence phenotypes in a phenomenon called epistasis. For example, rabbit fur can be black or brown depending on whether the animal is homozygous dominant or heterozygous at a TYRP1 locus. However, if the rabbit is also homozygous recessive at a locus on the tyrosinase gene (TYR), it will have an unshaded coat that appears white, regardless of its TYRP1...
02:13Overview of Transposition and Recombination
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
02:53Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
02:50Transgenic Plants
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
01:25Cell Signaling in Plants
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...