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Los genes CDC16 y CDC27 de la levadura restringen la replicación del ADN a una vez por ciclo celular
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, USA.
Cell
|April 5, 1996
Resumen
Los nuevos genes CDC16 y CDC27 en la levadura limitan la replicación del ADN. Los mutantes superreplican el ADN hasta 8C dentro de un ciclo celular, destacando su papel en el control del ciclo celular.
Área de la Ciencia:
- Biología Molecular Biología Molecular
- Biología celular Biología celular.
- Genética La genética.
Sus antecedentes:
- La replicación del ADN debe estar estrictamente regulada para que ocurra solo una vez por ciclo celular.
- El sistema de control del ciclo celular asegura la estabilidad genómica mediante la prevención de la sobre-replicación del ADN.
- Comprender los genes involucrados en el control de la replicación es crucial para la investigación del ciclo celular.
Objetivo del estudio:
- Para identificar genes en Saccharomyces cerevisiae que regulan la replicación del ADN.
- Investigar los mecanismos por los cuales se produce la sobre-replicación del ADN.
- Para caracterizar nuevos alelos condicionales que afectan a la replicación del ADN.
Principales métodos:
- Cribado genético para mutaciones de sobre-replicación del ADN en S. cerevisiae.
- Análisis del contenido de ADN (por ejemplo, acumulación de ADN 8C).
- Análisis del ciclo celular, incluyendo el monitoreo de la mitosis y START.
- Investigar la activación del punto de control (por ejemplo, el punto de control MEC1).
- Evaluación de la actividad de la cinasa dependiente de la ciclina (por ejemplo, Clb2-Cdc28).
Principales resultados:
- CDC16 y CDC27 fueron identificados como genes esenciales para limitar la replicación del ADN a una vez por ciclo celular.
- Una pantalla identificó nuevos alelos condicionales que causan la sobre replicación del ADN (hasta 8C).
- La sobrereplicación ocurrió dentro de un solo ciclo celular, involucrando a todos los cromosomas, y podría desencadenar el punto de control MEC1.
- La actividad elevada de Clb2-Cdc28 persistió en las células con sobre replicación.
- Estos hallazgos diferencian las funciones CDC16/CDC27 de los mutantes que causan sobre-replicación a través de la mitosis aberrante.
Conclusiones:
- CDC16 y CDC27 juegan un papel crítico en la prevención de la sobre replicación del ADN en S. cerevisiae.
- Estos genes pueden funcionar regulando la proteólisis dirigida de los iniciadores de la fase S.
- El estudio proporciona nuevos conocimientos sobre los mecanismos moleculares del control del ciclo celular y la fidelidad de la replicación del ADN.
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