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Interacción de la óxido nítrico sintasa con la proteína de densidad postsináptica PSD-95 y la alfa1-sintropina

J E Brenman¹, D S Chao, S H Gee

¹Department of Physiology, School of Medicine, University of California at San Fancisco, California 94143, USA.

|March 8, 1996

Resumen

La sintasa de óxido nítrico neuronal (nNOS) interactúa con las proteínas PSD-95 y PSD-93 a través de su motivo PDZ, formando complejos sinápticos cruciales en el cerebro y el músculo. Esta interacción es vital para la localización y función de las nNOS en las sinapsis.

Área de la Ciencia:

La neurociencia es la neurociencia.
Biología Molecular Biología Molecular
Biología celular Biología celular.

Sus antecedentes:

La sintasa neuronal de óxido nítrico (nNOS) está localizada en las uniones sinápticas en el cerebro y las placas finales motoras del músculo esquelético.
nNOS juega un papel en la señalización neuronal y la función muscular.

Objetivo del estudio:

Para investigar los mecanismos moleculares subyacentes a la localización sináptica de nNOS.
Para identificar las proteínas que interactúan con nNOS en los sitios sinápticos.

Principales métodos:

Los ensayos de coinmunoprecipitación detectan las interacciones proteína-proteína.
Análisis de las isoformas nNOS que carecen de dominios PDZ en ratones mutantes.

Principales resultados:

nNOS interactúa directamente con la proteína de densidad postsináptica-95 (PSD-95) y PSD-93 a través de su motivo PDZ N-terminal.
nNOS y PSD-95 forman un complejo en el cerebelo.
Las interacciones del dominio PDZ también median la unión de nNOS a la sintropina del músculo esquelético.
Los nNOS que carecen de dominios PDZ no se asocian con la PSD-95 y el sarcolema del músculo esquelético.

Conclusiones:

Las interacciones mediadas por el dominio PDZ son esenciales para la localización sináptica de nNOS tanto en el cerebro como en el músculo esquelético.
Estas interacciones son críticas para la formación de complejos de señalización macromoleculares en las sinapsis.

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