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Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina
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Requisito para la transmisión sináptica colinérgica en la propagación de ondas retinianas espontáneas
M B Feller1, D P Wellis, D Stellwagen
1Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, 94720-3200, USA. marla@violet.berkeley.edu
Science (New York, N.Y.)
|May 24, 1996
Resumen
Las ondas retinianas espontáneas, cruciales para el desarrollo temprano, son impulsadas por las células amacrinas colinérgicas que activan los receptores nicotínicos en las células ganglionares. Este sistema colinérgico es esencial para la propagación de ondas antes de que surja la visión.
Área de la Ciencia:
- La neurociencia es la neurociencia.
- Biología del desarrollo Biología del desarrollo.
- Fisiología de la retina Fisiología de la retina
Sus antecedentes:
- Las retinas en desarrollo exhiben actividad neuronal espontánea como ondas de potenciales de acción semanas antes de la visión funcional.
- Se cree que estas primeras ondas retinianas juegan un papel crítico en la formación de los circuitos neuronales.
Objetivo del estudio:
- Investigar los mecanismos celulares y sinápticos subyacentes a la actividad de ondas espontáneas en el desarrollo de la retina.
- Para identificar la fuente de entrada sináptica que impulsa estas primeras ondas retinianas.
Principales métodos:
- Se utilizaron imágenes ópticas para visualizar los patrones espaciales de la actividad neuronal durante las ondas retinales.
- Se realizaron grabaciones de células enteras para evaluar el papel de la activación sináptica y los receptores específicos.
- Inmunohistoquímica con anticuerpos contra la colina acetiltransferasa identificó poblaciones de células colinérgicas.
Principales resultados:
- Las ondas retinianas consisten en dominios de actividad de mosaico espacialmente restringidos a través de la capa de células ganglionares retinianas.
- La generación de ondas requiere la activación sináptica de los receptores de acetilcolina nicotínica neuronal en las células ganglionares.
- Las células amacrinas colinérgicas, uniformemente distribuidas, fueron las únicas células colinérgicas identificadas en retinas inmaduras.
Conclusiones:
- Las células amacrinas colinérgicas representan la principal fuente de entrada sináptica a las células ganglionares de la retina en el desarrollo de la retina.
- La actividad de estas células amacrinas colinérgicas es indispensable para la propagación de ondas retinianas espontáneas.
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